XB-CLONE-3063312
Clone Name: IMAGE:7845464
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Gene:
ubxn6
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528379 [+]
ccagctttgctccaatatgaagaaattccttgacggattaaagagtgatataaaattcaagggtgcaggaccgggacacaaactgagcgaagataccagg
gaaaatgtggctaaagttacaaaagaacaagttaaaccacgtaaacctcccacaggggaggcacagatagcagcggcttctgctgcaatggcgagaatgg
aatcgttgcaggggcggaccaaggtgcagtccaaggatacaacaacaggaagaaaagagatgattgaacagaaagaagagtctaatgtgggtccaaagga
tattggtaccacaagaaagaagtccagttctgtttgtactgtcctgttcagatgcccactcactgatgaactgctgaggaaagaggagagggagggccac
attagaaatgttttacagaggttgtccaccacagatcccacatctgctgccattctgaagattcacacatataacaaagacagagaaaaagtgaaacttg
gaactgagactatagctaaatacatgaataatataatcagtcaccctgaagaagagaagtattgcagaatcaaactgtcaaacaaggtgttccaggagaa
aatcagctgtctagagggttcccatgaattctttgaggctgttggctttgaaaagaagacaatgcctgggcaagatttacaagaagaattttttgttctg
ggtggtgatgcactgaaaaacctagatgcattacatgggcattgtgatgcattgctgagtgcagagcctttgagactaagtctggagaggcaattgcgta
ttttcatgccatccattgaagctgcacactttgaccttcctgatgac
3' EST: DT528378 [+]atgcaaatacccttttattcaaaacaaatcctctagatcaatagagtttaatggagaaatacatttgttgcgaaatacattgccttatcataactaaact
gtttcttgcttattggtttatctgaaaagctgctttggtacagatcctggtcttttatttaaccaaaggttaacctataacctgtgggcagttgtggcta
aaaacaaaaccataattgaatgttccctgctgattgattgcctacctgtatctgaaatgcaacatgccttgaaaactaatgactccaggcaagcactttt
ttgttttctttccaaaatgaatatcatcaggcgggacatgtagtgatatttcctatgtgagttgctctacctttgagagaagctctggctttattacact
gactgaactctgccctcccccagcctccacatcagccatgacagcctcatcccagcgaaaggtcagtagtgcagatggtaccagcccacactctatcaat
gtagcctgctcattctctactttgtgaccccctggagccagaagttcaaaaggaagccaatcattttgcagctgctcacgaagaaaatcaaacaaagcag
acacccgttcccgagcaaagaatatgccctgcaagatgtatccatctggcaatttgattctcagcacagtgtaattgtatttcttcatttctctctgttc
ttcacgctctcgcattgctttggttctgagcactgcattcctctccaccatttctggcctttctctttgttccctctttatctcttctgcagtcaggttg
aaaaaatcct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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