XB-CLONE-3063125
Clone Name: IMAGE:7845528
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Gene:
sorbs1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528503 [+]
agaaactcaagacataatagaaaaaggccgtgtttcaaatcagaaatgattagaatgatcctgttattgccaaanctgggctccancttttttacaaaaa
agatgatgatgcaagaaagtataatctttataatctgcctatcaaagcaatctcccaaactgtaaacacctgtaaaataaacacatttatgaaaaaaaaa
aaaaaaagtgagggcaacattgtaagtttatagcttatggtcataaaagaaaaaaaaaaaaaaaagattgagaatgttcttttgtcataacaagatatat
ttaatataagatgactatttttttgctattgcttttatacagatttattaacaattgcagctgcgttggcatgactagcaatatgttttgatatattacc
catgttactggtattaaagttttttttttttctttttccatgtacacagtacatccacattacaaatggttcataagagacaaactgtaaatatgtatat
ttgactttgtaaagaacatgtatgttgtatcttgtatagaaaaaaagagtaatttaagtggactgatggatagagaaaagcataaaattcaggaaaacga
agtcttgaatttaatttaatgaacgattttgttaacgacacaaggaaaagttaagcacagaatcattggacacatgaggaaatgacactgaacacctttt
agcaaacatatccattgttttattaatgtttttttctttcaacagaatttttagccaccatatttagagttatgttaccatttggctaattcacaataat
gctttccttacc
3' EST: DT528502 [+]catgctttaatttattaggcttcaaaatataatgcactgcaatatgaaaacaacacaacataacttgaaaagtgactggtatggtgataactgaacaatg
ctgttaaagtgtattggattatacggttgggtaaggaaagcattattgtgaattagccaaatggtaacataactctaaatatggtggctaaaaattctgt
tgaaagaaaaaaacattaataaaacaatggatatgtttgctaaaaggtgttcagtgtcatttcctcatgtgtccaatgattctgtgcttaacttttcctt
gtgtcgttaacaaaatcgttcattaaattaaattcaagacttcgttttcctgaattttatgcttttctctatccatcagtccacttaaattactcttttt
ttctatacaagatacaacatacatgttctttacaaagtcaaatatacatatttacagtttgtctcttatgaaccatttgtaatgtggatgtactgtgtac
atggaaaaagaaaaaaaaaaaactttaataccagtaacatgggtaatatatcaaaacatattgctagtcatgccaacgcagctgcaattgttaataaatc
tgtataaaagcaatagcaaaaaaatagtcatcttatattaaatatatcttgttatgacaaaagaacattctcaatcttttttttttttttttcttttatg
accataagctataaacttacaatgttgccctcacttttttttttttttttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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