XB-CLONE-3062915
Clone Name: IMAGE:7845929
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Gene:
crisp1.7
,
cox4i2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528641 [+]
cacagtttcagatgagatcccgggagcagatgggccgtctcctattatgggatgtggccggaattacagcttgtctgctcgttctgcagggagttctatt
aagcacgaaacgacttcatacgaagaggagaatggtttagagtacagaacgatcacaatttcagatcagatccatggagcagattccctcagccctaatg
aggagtttagtgatgggcgcgtggccacatacagtcagtccctcactaaccaatccccactgccattaaagctcctatacaagtgccctgtgtgcaacag
atcattcgacagtaagaggggagccgttcggcaccataagagaagccacgagttagaaaagaaattttcctgctccgagtgtaagaaaagctttaccgac
cgctccgccctcaaaacgcactacaaaatccacacgggagagaaaccgttttcgtgcgccgagtgcgggaaatcgtttttatacaacaaagacctgaaaa
tccactacaggatccacacgggggagaaacccttttcttgtacagaatgcgggaaatgcttcgcttacagctcggacctcaaaatccactggcggcttca
caccggggataagcccttctcttgctccgaatgcggcaaatgtttcacccaccgctcgggcctaaagagccacgaacgctaccacacaggggacaagtac
acgtgtgccgagtgcgggaagtgcttcgtacatggctcgcagctaaagagacaccttaggatccactcaggggagaagccgttctcttgctctgaatgtg
ggaaatgtttctataacagcttcgaactcacccgacacttccgcactcacacaggg
3' EST: DT528640 [+]gcaagaggtgtttacttttattgggaaaatttggcttttgaacaggaggaaaacatttcccatagtgaattctgagttgctgaataggagaacctctgac
aatcagagctgagagttactttacgttcccatcgcagattcaatctcctcttggaaggaagaagcatcgaatgaagtgtttccctcgtccaggggaggag
cctcatttcccctgtgacacttgagatggccagtgagatgggagcggcgggcaaagcactgagcacacacagggcagctgaatggtttctcccctgtgtg
agtgcggaagtgtcgggtgagttcgaagctgttatagaaacatttcccacattcagagcaagagaacggcttctcccctgagtggatcctaaggtgtctc
tttagctgcgagccatgtacgaagcacttcccgcactcggcacacgtgtacttgtcccctgtgtggtagcgttcgtggctctttaggcccgagcggtggg
tgaaacatttgccgcattcggagcaagagaagggcttatccccggtgtgaagccgccagtggattttgaggtccgagctgtaagcgaagcatttcccgca
ttctgtacaagaaaagggtttctcccccgtgtggatcctgtagtggattttcaggtctttgttgtataaaaacgatttcccgcactcggcgcacgaaaac
ggtttctctcccgtgtggattttgtagtgcgttttgagggcggagcggtcggtaaagcttttcttacactcggagcagggaaatttcttttctaactcgt
ggcttctcttatggtgccgaac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: