XB-CLONE-3062875
Clone Name: IMAGE:7845837
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Gene:
cdc42
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122003 [+]
CTGACCTTCACACCAGAACATTTCAGCCATGCAGACAATTAAATGTGTAGTCGTTGGTGATGGTGCTGTGGGTAAAACATGTCTCCTTATTTCTTACACA
ACAAACAAGTTCCCTTCTGAGTATGTACCAACGGTTTTTGATAATTATGCCGTAACAGTTATGATTGGAGGGGAACCATATACCCTAGGGTTATTTGATA
CTGCAGGACAGGAAGATTATGATAGATTACGACCTCTTAGCTATCCACAGACAGATGTGTTTCTAGTTTGTTTCTCCGTCGTGTCTCCATCTTCATTTGA
AAATGTGAAAGAAAAGTGGGTACCTGAAATCACTCATCACTGTCCCAAAACTCCATTTCTGCTGGTTGGCACCCAGATAGATTTAAGAGATGATCCTTCA
ACAATTGAGAAACTGGCAAAAAACAAACAGAAACCAATCACTCCAGAGACAGCTGAGAAACTGGCCCGTGACTTAAAGGCAGTAAAATATGTGGAGTGTT
CTGCACTTACGCAGAAAGGCCTTAAGAACGTATTTGACGAAGCGATATTGGCCGCCTTGGAACCCCCGGAGCCCAAGAAGAAACGCAAGTGTAGGCTGCT
ATGAATATTCCTCCGTGGTCCCCTTTTTTGCAGTGCTGGTGTCGACGTCAAACTAAAAGCAAAGTTAAAATCAAAACTAAGTATAAACTGTTAAATTTGT
TTTGCAATAATGCCAGATGCTCTGCACTCATTACCATTCAGAATAGTGGGGTGTCTGAAGACAAAACCCAGCAACCTATTGAATTCAGTATAAATATTAG
TAGAGCGAATATGTATCTGCAGAAAAAAACACTGATTACTAGTTTTATTCATTTTTTTTTTTATTGTGTTATTTACTATTTCTAATGTCTTATATTAAGA
GCAAGGCATGCTTCATACATAACAGACTAATTTTTAAGCTCTGTTTTTTTTCCAGCCCAACTCTTTCCCCAAGTTGCTGGGAATCAGGAATTCCTTCTCT
GCTCTCCTGCAAAAAGTACACAAGTTGGCTAGAGAACAGGTTTAAATATAAAACGATTGAATCTCAGGACTATTAAAGGTCTCCCAGAGCCCAAATATAG
AAAAAGCCATTGGCTTTTATATCTCTAGCCACTGCGGCATGTAGCGTTGCACACAGAATGCTACTTCTGTTTTTGGGTCAGCTGCTTCATTTGGGAAGCA
CTAGGGAATAGTGACCATGAGTAACCAAATATTTGCCAGTAAATGATACTTCTTACATCTTTGAATTCCCTTGTTCTTTAAGGGGCTACAGCTGCATTGC
AGAGCAATGCCTTGGAAGCTTTTGTGATCTCAAGGAGGCCGATGTACTTTTTGACAGTGTCTTGGCAGGTTATATCAAACCAAAATTTAGATTTTTGAAA
ATGTTTTCCAATCCGGATAATTGAATTTGATTCCAGTTAAACATACTAGTAGTTAGCTACCCCTTAAAGTTCTGACAAACTATACATGTGCCTAATGTTT
TTCCTGTCTGTTTTGTTTAGTTTGATACATGAGGGACCAGTCAAAAAAATCCCGTTTTATGTACATTTTGCATGCAAGGTCTAAGAATCCTTATTTTAGG
GTTCCTGTCTAAAACATAATGTAAAAATAGACACCAGTGTTAATAAGGACTTTACCAGAACCCACTTTTTTTCAGAATTTTTAATATGTAGGAAATATAT
TCCATCCTCCTGGATAACACATGTAGACTCATTAGTGTTGAACCCATGCTTTGTTTCATGTCTGAGTTCTTTGTATATGCATACTTTTCAGATGTATTAA
ACATAAAATACCTTCACTTAAAAAAAAAAAAAAAAAA
5' EST: DT528667 [+]ctgaccttcacaccagaacatttcagccatgcagacaattaaatgtgtagtcgttggtgatggtgctgtgggtaaaacatgtctccttatttcttacaca
acaaacaagttcccttctgagtatgtaccaacggtttttgataattatgccgtaacagttatgattggaggggaaccatataccctagggttatttgata
ctgcaggacaggaagattatgatagattacgacctcttagctatccacagacagatgtgtttctagtttgtttctccgtcgtgtctccatcttcatttga
aaatgtgaaagaaaagtgggtacctgaaatcactcatcactgtcccaaaactccatttctgctggttggcacccagatagatttaagagatgatccttca
acaattgagaaactggcaaaaaacaaacagaaaccaatcactccagagacagctgagaaactggcccgtgacttaaaggcagtaaaatatgtggagtgtt
ctgcacttacgcagaaaggccttaagaacgtatttgacgaagcgatattggccgccttggaacccccggagcccaagaagaaacgcaagtgtaggctgct
atgaatattcctccgtggtccccttttttgcagtgctggtgtcgacgtcaaactaaaagcaaagttaaaatcaaaactaagtataaactgttaaatttgt
tttgcaataatgccagatgctctgcactcattaccattcagaatagtgnggtgtctgaagacaaaacccagcaacctattgaattcagtataaatattag
tagagcgaatatgtatctgcagaaaaaacactgattactagttttattcatttttttttttat
3' EST: DT528666 [+]aagtgaaggtattttatgtttaatacatctgaaaagtatgcatatacaaagaactcagacatgaaacaaagcatgggttcaacactaatgagtctacatg
tgttatccaggaggatggaatatatttcctacatattaaaaattctgaaaaaaagtgggttctggtaaagtccttattaacactggtgtctatttttaca
ttatgttttagacaggaaccctaaaataaggattcttagaccttgcatgcaaaatgtacataaaacgggatttttttgactggtccctcatgtatcaaac
taaacaaaacagacaggaaaaacattaggcacatgtatagtttgtcagaactttaaggggtagctaactactagtatgtttaactggaatcaaattcaat
tatccggattggaaaacattttcaaaaatctaaattttggtttgatataacctgccaagacactgtcaaaaagtacatcggcctccttgagatcacaaaa
gcttccaaggcattgctctgcaatgcagctgtagccccttaaagaacaagggaattcaaagatgtaagaagtatcatttactggcaaatatttggttact
catggtcactattccctagtgcttcccaaatgaagcagctgacccaaaaacagaagtagcattctgtgtgcaacgctacatgccgcagtggctagagata
taaaagccaatggctttttctatatttgggctctgggagacctttaatagtcctgagattcaatcgttttatatttaaacctgttctctagccaacttgt
gtactttttgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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