XB-CLONE-3062838
Clone Name: IMAGE:7846079
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Gene:
optn
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528691 [+]
atccatcgattcgggaaaatggacatcaggatgcctcatgtgatgtttttagcatgaagaatgatgcagaaatgttggaacagataaaacagcttctaat
ggaaaataataatctaaaagaaaccatgaagcaaatgaatcaagagatgaaagaacgccttgaagagctcctgaagagacacaatcagcatttgctagac
ttgaacagcgaaaatgaattgttgaggaaggaattaaacagtttaagagaaaaaaatgccacatctaatcaaggtataccagtttgctctaacccagagg
agacaagtgaaagcaaacagctgaagactcagttaatgcgtttacaggcagagaaagctgacctgcttggtctaatatcagagttgcagctgaaactcag
cggtttttctaaagactcatttgtggaaatcagaatttctgaaaaggagtctggagaaataatacatgaagaaaaagcaaataaaacactaccagactat
gatgtgtcatatagcaggacaaactctgtcaaggaagaagggagtggcactgaaccagaagaggtggctatcagcaggctccttcgcagtctgagagagg
aaactcaaaaagtggagagactagagaaggaattaagtgaacataaaaggttggcagaactaaaagaaaaaacctccagattctgtgacaaggacatgca
aacagagcaggaaaaacagaatcagtctgaagagattatttccagtgaagtagatatattaaagcagaaagtgaagtcactgaataaagagcttcaggaa
accaatgataagttnaacgaggctaaacagttaaagaacagtct
3' EST: DT528690 [+]aaagagagaaaatctgtttattaaacttaagtcattaaagttcagtgtaaggtactacatgacattaggcatcacaggtcataacagtaggtgttcactt
gtgattttttttttgctaatatataataggataaccccagagtgcaaatcatattagcggaactatttttagtaccttgatgtttaaagcaaagatcaga
gctatttaagaacaaatctgtgtttggtaaagtgcttggtgatattatatatacaattctaaatgcaaaggactctgtgttgcatttagtggcacaaatg
gagatcttgtgcttaggtgatacagtccatgacatgaatctgcagcgtatctatatctggaacagtcaggttacattttgggcatgtatacacagtaata
cttggctgtcccatgttttctgctcctcttggcacaagataaggacccccgctgacattctctaatctgcaactatggcgcctctggagctgttctattg
actgcctccccatcatttctgccttaagtttttcactttcttgtatcatatatgccagacgagcttctaactgctccttctcttgatgaatgttctctct
agctgatctttcggcatggaaatctgcacaatagacgtcaatctgagcacgaagcagaccaattgtttctatatcctccttgtgttttgcgtccatttgt
ctaatttcatcaatttcaaattgttttttcgccagtgccttttcacatgcatccagcttttcaaccagatcgttaaactcaactttggaaactttttcac
tctcgcgtatccttgtttcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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