XB-CLONE-3062826
Clone Name: IMAGE:7845889
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Gene:
csde1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528699 [+]
aaaccaagtctccagctgctccaggccagagtccatctggaagtgtgtgttatgaacgaaatggggaggtgttttatttgacatacacaccagaagatgt
agaagggagtgtccatctagaaactggagataaaataagctttattattgaaactaataagcatactggtgcagttagcgcacgaaatataatgattttg
atgaaaaggaagcaaatgcgttgccagggagttgtttgtgccatgaaggaagccttcgggttcattgaaagaggagatgttgtcaaagagatattctttc
actatagtgaatttaaaggtgaccttgaagccttacaacctggagatgatgtggaatttacaatcaaggatcgaaatggtaaagaagtggcaacagacgt
ccggcttctgccccaaggaactgttatctttgaagatattagtattgaacattttgaaggaactgttaccaaagttatccctaaagtacccaacaaaaat
cagaatgaccctttaccaggccgtataaaagtagattttgttattcctaaagagctgccatttggtgacaaagacacaaaatctaaggtcacccttctgg
aaggcgatcgtgttcgtttcaacatatccacagatcgacgagacaagttggaacgcgctacaaacattgaatttcttcctgatacctttcagtataccaa
tgagtctagagaaatggacatgttgtctgcacagagaaatcatgccataaggattaaaaagcttcctaaaggacagtgtcatttcacacacagtcagaac
atcgctttattggtcttgtcga
3' EST: DT528698 [+]caaattaattggtaaaactttattctatttttcaattttttttgccattaaacagaaaaactggagccaagtatcattttataaaggtaatgtgagtctc
caaaaaaaaaaaaaaacaaaaaaaaaaaaatactttccctctttccgtagaaagtggcttgatcttttttgcttaaaataaaaagggcaacttaatttaa
tattttatgcgagcgggtaatggaccgaaacaaataaaatagggctggggggagggtagtttaaaaaaaaaaaattatatattaggtaccagctttctaa
atttatcttgatgcgaatgaccaatataactatcaaaagactaaatcttatgataaatcggtgctgtaaacaaaaacaaaaaaaaaaacaatgtttcagg
cacatttttcttccttttaaatccattaatgtttcaaaagctgcagtgtcaaagcagcaaaaacaaaaaaacaaaacaaccgtgcattttcgtattaatt
taacacacatggcacatttcaggcttgttgtactctgcacttccaaagtcacttgttaacacaaaaaccaaaataaattttgaagaaatgcattcaaaaa
ctaacagcgtgtgaatgcagagttaattaatcccctctgaaaatgtgaatgtttggctgtcactggaattagaaaatgcttcattttttatcattgctta
gggaacactgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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