XB-CLONE-3062713
Clone Name: IMAGE:7845851
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Gene:
trappc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528774 [+]
acaatattttacattgcaattacaatacagcaaacatacatacaataataaatgttccaggtctgcacaagaaaataagaggtattgtatttttcaaata
ggcttaatattgttagcattttttggaatgcatcagaatactcatcctccctgtaaagaaatatcacaggattcagtgatatcctgtcttaccgtacagt
tttactactacaatgaacctaataacgttaaaggacctcaaactataacataagatcatttgatatctcattttaactgtgctggcagccaaactaattc
agctatggccttttagaactgctaaatgttttcattcagaaaaattgcaatagagctcatatagctaaaaaactgtaaacagcacacaaagcagtgacat
gcagacctcaggcttctgacaaatgtcagagggagtcactatttattgggcctttgagggggctgtttgggcctctgtgcacttcagacttagtttggat
ctggcagacctccattcccgtaaccgctccatgtacctcagacacaatgattttgtattgaataacaatgtaagtggcaacagggttgttttgaccagca
cagtttatacaggatggcatttcagaagccttactgtacagtttttcatctacataaactctatggcaatgattagaaacaacaaaaccagaagcagtag
aagatgtttcataacttaatcaaacaatgccattatttcacactacttcagacctggcataatccattggagcta
3' EST: DT528773 [+]aaagccgggagagagagatctacatttgaagataatgtccgggagtttctatttcgtcattgtaggccaccatgataaccctgtgtttgagatggaattt
ctgccccagggcaaaacagaatcaaaggatgatcatcgtcatctaaaccagtttatagctcatgctgcccttgacctggttgatgaaaacatgtggctgt
ccaacaatatgtacctcaagacagtagacaaatttaatgaatggtttgtctctgcttttgtcactgcaggacatatgagatttataatgctacatgatgt
acgccaagaagatggcataaaaaatttcttcaacgaagcctatgatttatacataaagttcgcaatgaacccattctatgaaataaactcgcctttacga
tcaactgcatttgacaggaaaatacaatttctgggaaagaaacacttgttaagttaatagaagaacagtaaagctattcattggtaataaaaggaatggt
aatttactctgataatgactttatgagtattctgagtgaatactctatacgccattccttaaagagggtcctatgtagagggaatataccagcctatctc
tgctgtttgcatgaatgtgactacacttgactacaaatactgatacaaagttgattccttgatgaaacaaacatgaaaatatctaaacttttgccctacg
attagttattaaaccgtattgtttcattttttataggctactgagagagagagtacttaactaaactgtctgaacatttgcctttagctatccttttatt
gcagtggctaaatacactagaaactgctncaaactagaaatagtgaggctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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