XB-CLONE-3062678
Clone Name: IMAGE:7845807
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Gene:
snta1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528797 [+]
ctcagacactttattgtttattgtacacctccaaagggcaagtaaacacnctccgtttctttccaaacaatcaggatcatgaaacgtcctcccatatata
tatatatctatatccacacgctaaatactttgtaaaataataggttcaagtccagaataaacccccccaaatgcccttattctaatgcagcttaaaaagt
tgtgccgggtagcttaatttcaataattaataaaactagatacatatctgcctctatgatcatttatgaatatatattaatatatttatacaaacgccct
aacctgtgctccgaaggggaaacaattagaccagattgatagctgattgtagagcgggttgtgctgctttataacaacatggcgtggagcagatattggg
catttaaaggcacagtacttttgtttttccccaaaaacccactgtatatcagagagactgacctggattctccctgaagccatggcattaaacctttgaa
aaagaaatatatatatatataaggcactataattcatggttagggaatcggcagtttacaaagaataaagacgagaggcttcacatttccttgccattaa
ccgtatccagacatagtgacatagaagtggttaattctggccatttatggtgtgtaaatacaaatattatttgtgtgacattttggaaagctgtccactt
tggactaactctccagcaccccctagggttggttttggttagcgctcggccgcgaggtccaagattgccgccactttcagcgttctaggtgaataggccc
agtcgggacactttggccgacaggaaggagtgaatgatgaagacaatggtttt
3' EST: DT528796 [+]ttagggcgagaggcctcccggagcgggctgctggagctgcgctgtcccggggagcgctgggtgcgggtactgctgctgctcggagaggagactgtgactt
tcaccccggagccggagcccggggcagcggaggagaaggcgctgaacggcgcggagccggcggggggggtcccggagtctctcattaacctgcgccggac
tgtcagggtgctcaagcaggatgcgggggggctgggggtcagcatcaagggtggccgggagaacaagatgcccatcctcatctccaagattttcaaaggc
ctggctgctgacttgacagggtcattatatgtgggggatgcaatcctgtctgttaatggggtagacctgtcagaggctactcatgatgacgctgtgcagg
ctctgaagaagactggaaaggaggtggtgctggaagtaaagtacatgaaggaaatctcaccgtatttcaagggctctcccccgagtggttcacctgctgc
ccctttacagaagagggggtcccccgcgctcccacccaaggaatacaaagatggaaagagcattccgctgaagatgtgctacgtatcccggagattccta
ccaacggacaccgagcccaggtacctcgagatctgctcagcagacggaagggatgtgctgttctttcgggcaaaggatgaaggtacagcccagtcctggt
ttaatgccatccacaccaatgccagcgccctggcgccccgtgtgcgggaggatgtgaagcagctattgggcaaagacatcaaacagatgggctggctcac
tgagcagctccccccagatggaaag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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