XB-CLONE-3062639
Clone Name: IMAGE:7846049
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Gene:
mypn
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528823 [+]
acctgtgtttgctaagcctaaactagaccctctgaagttacagcagcttcataaccaggttatgattgaacagcaacaacagcagccgtcatcaccagtt
tccttggcaagggacacaacggttagtacgcctgtacaaaactctactggttctcagccttacatgtttgcccgaccccagcagtttatttctggctcag
atccaatcaatagcacgtcggtgtctcctaaatatcccttttccactactggcacatcgacgataagaccatggacttcatgcagaggaaatattccaga
agtctacactgaggttggatcagctacagccaaaaatgaaccttccactgctatggagtccatcttgtcttctccaccttctgcaccacaaagacaattt
caggcacagactgtgtctcctactccaatatctcctccacggattcagaatcctgctgcatttctcagtgcagttctcccatcattgccatcttcaccac
ctcctccttccacaaatgcgatggggctgccaaaaacttcatcattgtctgttcctggagctgcaaagaaaaatgtaaaaacagtgcggtttggccctga
agatcaaattcttgagaataagaattctgttgtaaaagagttggagagaaagctgtcatttagagataacactccacaatatattccacggcaacagggg
gagcgggagttaacccatggtgtgctgccttatggactaatggaaaagaaaatttcaatggaatacaaaatatccagctttgagcagaggctgatgaaat
gaatagagtttcgtttgnagcgcaccccggtggatgaatcggatgatgaagttcagcacgatgaaatcccaactggaaagtgca
3' EST: DT528822 [+]atgcaacatgttgtaactttattagggaatacatcccattttcacttcctataaacaaaccgccatttgttaaatagcaagagatggcacttttagttta
aaccctctactgtataagcaaccttgttttaacaaagaatctggtaaaatacaaataatgcacatttatatattaatataacctctataggtctttactt
tcttttctttgtggacataaatatttaaatagggtacttatacaacatcaaacagatcacactgcatcagtgtcagtgccacgggcatacagatcactga
gtactgctggcgcgtctgggctacatatactgcaaacctatgttaacctgttcactgccagaaagggctgggcccctctagtggcactgaaacagtacta
cctggcagttagattattttatattggcaaaacactgaagccaaacagggatctttatccccattaaaaatttgatttgtaaacgttcaagctgtatttt
cttcacagttttattttgggttttgacgtcggttccattcacagctcttcactttctaccacttcccgtgtagacaatgagtaattcagcggtgttacta
caggaggatgagagctgatggcatccaaagctgaaaacatagaataccgagaacctgttgatcgggtctttttgattggctggggaatctgctggtacca
ctgagcataaatatcaagtcttgcagtacaggatacaatccctgcttcgttctttgctgacagtgtgtaccatcctgcatcttcttttctggcaggctgg
atgaggaggcacacgtaaacagtgttatcttgatgcatgctta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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