XB-CLONE-3062284
Clone Name: IMAGE:7845938
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Gene:
thap4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121669 [+]
CCTGTTTCCGGCTCGGTGGGTGACGACATCTTGTTGTACGGATTAGGCTAGTCATGGTGATATGCTGTGCTGCTCCGAGTTGTACCAATCGCCAAGGGAA
GGGAAAAAAGGGAGACGTATCCTTTCACAGGTTTCCTCTGAAGGATCATGTTCGTCTATCTCTCTGGACAGATGCATTACAACGTGATAATTGGACCCCT
GGACCATATTCTTTTCTCTGCAGTGACCACTTTAGTCCAGAGAGCTTTGTCCGGCGTATGCCAGACCAAACCCCACTTCTTACATCAAATGCTGTGCCCT
CCCTCTTTAAGAACTCTTGTGGAAGGAGAAAGAGAGGAACGGTGCTTCGTGTCTCGGGAGTAAGAAGGAAGAAAAAGGCCCCAAAAATAAGACCAGTCCT
TATTCAGGGGAATAATTCTGTTGAATTGCCTAGTGGAAATATTTGTGCTGAAAGCCAGAACAGTATTATTATTACTGATGATTTCAAAGACGCTGGCTGC
TTCCTTACCCATGATGTGTCACCTAATAGTTCCAGCGCGTGTAAATTTATTTCCTGCTTACACTCCTATAGCAGTTCAGCTAAGCCACCTCTTTCCCAGA
ATGGCACCAAAGTGAACCTTTTTTCTCAAATAATGGATGTCATCAAACCATCTCATACACCAACCAGTGAGGTCTTGCCCTATGCAACCAGTGGTGTTAC
CCCATCTGATGACAAAGATTCAGACAACAGCCATCTGTGTCGTGTAGAATTTAGTAATGATAAAGGATCTCAGATTATTCACACTGTCACTGCACAACAT
CCAGTCTCAATGGAGGACACTCACTGTGAAAACAAAGAGTCATCCACAGTAGAGACAGAGGACCCCAGTTCACAATCAGATATGCAAATCTGCCCTATTC
AGCCAGAAAATTACTCTGCCCTTTACCAAGACATACATTCCCTTCATTCTTACTGCTTTCAATCAAAACCACTTTCAGGACGTAGAAATCATCTAAAAAA
GAAGGGGAGAAATATGAAAGACAGAGGTGATCATTATTGCTGTACCCAGTCCTTTTTGGAACTGCAGTCTTCCCCTCCTCTGAATCCTGTGGTTTCTCAG
CTTGCTTGGATGTTGGGTACCTGGGTGTCTGAACCTGCTGGTGAGGGGCAATTCCCCACCATTCCACCTTTCCGCTACATGGAGGAAGCAACCATCACTC
ACGTGGGACAGCCAATGCTAAACTTTATGTTCTGTGCTTCTAATGCTGAGACTGGGAAGCCAATGCACAGAGAGTGTGGGTTCATCCGTATCAAACCTGG
TACCAATCACGTGGCCTTCATTAGTGCTCAGAATACAGGTGTTGTAGAGGTGGAAGAAGGGGAAGTTGAAGGAGAGCAGCTCAGTCTAACTTCAAATTCA
GTCAGCAGAATTAGCTTTGCCAAGGAGCCTCATGTCACACAGATATCCCGTAAGTTCCGCCTGACCCCAGAGGGCAAACTGGAGCAGACTGTATTTATGG
CCACCGCTTCACAGTCATTGGCTCCCCACTTGCATGTAACATACAGGAAGGTGACATCTTAAGGTTCAGGGCCATGATGCTGCAGGAACATCTGTACTGT
GCTGATTGCAAACCTATGTCAGAATTTCCATATCCAAATAAAATGGTATATTTTTCCAAAAAAAAAAAAAAAAAA
5' EST: DT529057 [+]cctgtttccggctcggtgggtgacgacatcttgttgtacggattaggctagtcatggtgatatgctgtgctgctccgagttgtaccaatcgccaagggaa
gggaaaaaagggagacgtatcctttcacaggtttcctctgaaggatcatgttcgtctatctctctggacagatgcattacaacgtgataattggacccct
ggaccatattcttttctctgcagtgaccactttagtccagagagctttgtccggcgtatgccagaccaaaccccacttcttacatcaaatgctgtgccct
ccctctttaagaactcttgtggaaggagaaagagaggaacggtgcttcgtgtctcgggagtaagaaggaagaaaaaggccccaaaaataagaccagtcct
tattcaggggaataattctgttgaattgcctagtggaaatatttgtgctgaaagccagaacagtattattattactgatgatttcaaagacgctggctgc
ttccttacccatgatgtgtcacctaatagttccagcgcgtgtaaatttatttcctgcttacactcctatagcagttcagctaagccacctctttcccaga
atggcaccaaagtgaaccttttttctcaaataatggatgtcatcaaaccatctcatacaccaaccagtgaggtcttgccctatgcaaccagtggtgttac
cccatctgatgacaaagattcagacaacagccatctgtgtcgtgtagaatttagtaatgataaaggatctcagattattcacactgtcactgcacaacat
ccagtctcaatggaggacactcactgtg
3' EST: DT529056 [+]ggaaattctgacatanggtttgcaatcagcacagtacagatgttcctgcagcatcatggccctgaaccttaagatgtcaccttcctgtatgttacatgca
agtggggagccaatgactgtgaagcggtggccataaatacagtctgctccagtttgccctctggggtcaggcggaacttacgggatatctgtgtgacatg
aggctccttggcaaagctaattctgctgactgaatttgaagttagactgagctgctctccttcaacttccccttcttccacctctacaacacctgtattc
tgagcactaatgaaggccacgtgattggtaccaggtttgatacggatgaacccacactctctgtgcattggcttcccagtctcagcattagaagcacaga
acataaagtttagcattggctgtcccacgtgagtgatggttgcttcctccatgtagcggaaaggtggaatggtggggaattgcccctcaccagcaggttc
agacacccaggtacccaacatccaagcaagctgagaaaccacaggattcagaggaggggaagactgcagttccaaaaaggactgggtacagcaataatga
tcacctctgtctttcatatttctccccttcttttttagatgatttctacgtcctgaaagtggttttgattgaaagcagtaagaatgaagggaatgtatgt
cttggtaaagggcagagtaattttctggctgaatagggcagatttgcatatctgattgtgaactggggtcctctgtctcta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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