XB-CLONE-3061973
Clone Name: IMAGE:7846491
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT529262 [+]
tttattgtctccaggcaatcattggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatcagagaattagatttatc
ttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcc
cctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcc
cctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactacca
tctggacttggtccacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctctt
ctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagttt
gatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcacagaagc
gtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccacaccaggaaggtcttgttttcattgtg
ccagataccacgggcatcag
3' EST: DT529261 [+]ttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctg
ctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattcagactggagttgacaacccaggccatcctttcat
catgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatcccattattgaggatcgtcacggtggttacaagcca
actgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgta
gcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacactccatcaaagctctcagcagtctgtctggggaatt
caaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgctt
ctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagaccttcctggtgtgggtaaatgaagaggaccatctga
gagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttcagaagattgaagagatctttaaacaagcaggaca
ccccttcatgtggaatgaacatctgggttatgtccttacctgcccatcaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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