XB-CLONE-3061810
Clone Name: IMAGE:7846171
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Gene:
ivd
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT529370 [+]
cgggaatatcggtggatgacactgtcaatgggctgaatgatgagcagaaacagctccggcagaccctgcacaggttttgccaggatcacctcaaccccgt
tgccaatgagattgatcagaagaataactttgcggagatgaggagcttttggaagaagcttggagaacttggggttcttggaattactgctccagttgag
tatggtggatctgctatgggctacctggagcatgttctagtagtggaggaaatatcccgggcctcagcagccgttgggctgagttatggggcgcactcca
acctctgtatcaaccagatagtccgcaatggaaacgaggcccagaaagagaagtatcttcccaagctgatcagtggggagtacattggagccctggctat
gagcgagccaaactccgggtctgacgtggtgtccatgagattgaaggcagagaagaaaggtgattattacgtcctgaatggcaataaattctggattacc
aatgggcccgatgctgatgtgctggtggtttatgcaaagacagacccttcagctcaacccgcctctcatggtatcactgctttcctggtggagaagggga
tgccagggttcagcaccgcacaaaaactggataagctggggatgaggggctctaacacctgtgagcttgtgtttgaggactgcaagattcctgagcagaa
tgtattaggccacttgggaaaaggagtgtatgtgctgatgagtggtttggacttggagaggctggtcttgtctgggggcccattgggcatcatgcaggct
gtgttagaccatgcaattccctatctgcacaccagagaggcctttgggcag
3' EST: DT529369 [+]gaaactattataaagtttattatagagataatcacaggcttgacaaaaaaatcatgtttccttcctataggcacaggatatgttccactgtcagacagca
atatcatcagttggtagaataagtattctataattctattgtataagtacattcaatgttggggggggggggcaaaaaccatctgacacaacacaaaata
tctccatagagttgtggtgcttgttgcaagaaaaaccttactatcaggagccgtctcatttacagttagtcttcaggatacctttcaactgagacatcat
cacaaaagcacaatgtctcttattcccatttagaatgttccattattactgggggtctgtatgataatagagagatttgctatcaacccaagtcctgtat
cacaccaagtcccagctgggcactgtactttattggtttctctgtgcggggttcttaaatatccatggcgcaaacatatgatgtgtctgtgacaaatatc
tgggcgtcccatgaacgaatactctgagtctgtacagcacgaggccatagtgagagatcctggttatttgtacagagcgttgaaggccctgcctatgagc
attctccgaacttcactggtgcctgccccaatctcatacagcttagcatcacgaaggaagcggcccatgggataatcgttgatgtacccattgcccccca
gacattggatgccatccagagccacctgtgtggcacactctgctgagtacaggatcaccccggcacagtcctttgaattgaaatgtccctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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