XB-CLONE-3061728
Clone Name: IMAGE:7846179
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Gene:
myoz1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT529424 [+]
caacctcctgtacctccaccaaaacctggcagtaagagcgctcctggaggccagtctggaactggaggaagtggtggaaaggattctggtgttgttgatg
gtagtcaaggaaaagagggacagtcaggtcaacaggatggaggaagtgggaagcagatcactgtctttaagacatacctctcaccctgggacaaagctat
gggcattgaccctaagcaaccaacacattttactgtcaatttactagagtatggcaaaaaggcagagctttgcaaatataagtcatttaacaggagtgct
atgccttttggtggatatgagaaagcatcgaagcttatgactttccagatgccagattttgatgccccccctccagagcctgagcctgtggttgtcatca
atcaagatatcaccaacagaccttctttcaacaggaccccaattggatggcttggatctggtgataacacaaactacagcattgatattgctgtgccagt
ggatggagaaacagaagaactctaaactcatcatacatccaaggaaccatgttagcagtctgttggtgtatgaaaactgacatatgtaaaaagttccact
ggcctctgcatcataacatattgcaaacacattggcggttgatccctgaaatatctgtggtacaagcaatgcattgcagtatgctgtgatgccatagcag
ccaatggcacaggtcatatctaaaccacctaagccaaatctttatatgccttttttaaaaaatggggcagatcacattagacccaactatgccacactct
aatgtgccttgttcaaaat
3' EST: DT529423 [+]taacattgcacaaatgaaaaatatcaaccatacagcatgactgaggagccaacagtaaacttatttggagactgtccttttatcgctgccacagtgcaca
aaacattaagcttttttccttccactatcctaccttgtctattccatttacctctgcccagttccaactctgactaacttccagctgaaaaaataccacc
ataacaaaaaacatttcataaacatacataaaggagctttgctgcattttgaacaaggcacattagagtgtggcatagttgggtctaatgtgatctgccc
cattttttaaaaaaggcatataaagatttggcttaggtggtttagatatgacctgtgccattggctgctatggcatcacagcatactgcaatgcattgct
tgtaccacagatatttcagggatcaaccgccaatgtgtttgcaatatgttatgatgcagaggccagtggaactttttacatatgtcagttttcatacacc
aacagactgctaacatggttccttggatgtatgatgagtttagagttcttctgtttctccatccactggcacagcaatatcaatgctgtagtttgtgtta
tcaccagatccaagccatccaattggggtcctgttgaaagaaggtctgttggtgatatcttgattgatgacaaccacaggctcaggctctggaggggggg
catcaaaatctggcatctggaaagtcataagcttcgatgctttctcatatccaccaaaaggcatagcactcctgttaaatgacttatatttgcaaagctc
tgcctttttgccatactctagtaaattgacagtaaaatgtgttggttgcttagggtcatgcccat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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