XB-CLONE-3061725
Clone Name: IMAGE:7846227
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Gene:
eef1g
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT529426 [+]
ggcatacaagcccctcattgctgctcagtattcggggttccccatcaaggttgcctcctctcctccagaattccagtttggagtaacgaataagacatca
gagttcctaaagaaattccccttaggcaaggtaccagcatttgagggcaacgacggtttttgcctttttgagagcagcgccattgctcattatgtgggca
atgatgagctccgaggaaccaatcgtttacaccaagctcaggtcatccagtggctcagcttctcagacagtcacattgtccctccggccagcgcatgggt
ctttcccactttagggatcatgcaatacaataagcaggccacagaacaagccaaggaggagataaaggctgtgctgggtgttttagattctcatctgaag
acacggacattcctagtgggcgagagggtcacgctggctgatatagcagttacttgctctcttctgtggctctataagcaggtcctggaaccatccttcc
gccagccatttggtaatgttacaaggtggtttgtgacctgtgtgaatcagccagaattccgtgctgtgttgggagaagtcaagctttgtgacaagatggc
acagtttgatgctaagaagtttgccgagatgcagcccaagaaagaaactcccaagaaagagaagccagcaaaggagccaaaaaaagaaaaggaggaaaag
aagaaggcaacaccagctcctgctcctgccccagcccctgaggatgatatggatgaatctganaaggctttagcagcagagcctaaatcanaggacccat
acgcacatctacctaaaagctcttttgtaatggatgagtttaaga
3' EST: DT529425 [+]ttgatttcagtttttttattttcggtttgctgtcttgttaagttaaaaggctctggatgttggtacggatgtgaaaaccagactctgcgcgtattttagg
gggacagagatcacctcctcctcatttgaagatcttgccctggttaaaggcctttcccacgtgcttgaagtctccctcccagcagaagtattctttgacc
agggttttgcactcctcactattgctgtccagctttctccaggtgtaggattcatagtcaatttgccagtcttcagagagtgtgaatgcaaggtcctggc
ccctgaacacccacacgccggagatggagctgtcattgttggtgccaaacaagatgacgctggcaaagccagtcttgcgcagtttgtctagacgctggaa
catacctgtaattaagttgcagctcatgaaggtttgtgtcagctcctcggggaatttgtattcagcgtaccagatggaccagccttccttgtcaaagtgc
tcccagaaataaggcagagctacagccagggtatcctcattagagtattttctcttaaactcatccattacaaaagagcttttaggtagatgtgcgtatg
ggtcctttgatttaggctctgctgctaaagccttttcagattcatccatatcatcctcaggggctggggcaggagcaggagctggtgttgccttcttctt
ttcctccttttctttttttggctcctttgctggcttctctttcttgggagtttctttcttgggctgcatctcggcaaacttcttagcatcaaactgtgcc
atcttgtcacaaagcttgacttctcccaacacagcacggaattctggctgattcacacaggtcacaaaccaccttgtaacattaccaaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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