XB-CLONE-3061534
Clone Name: IMAGE:7846387
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Gene:
MGC75753
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT529552 [+]
ttctctcttgactcatgatttcattttacaatgtacaatttcagagaccggagcgagaaaatgtcataaaagaaagacagaaatgacaataagagctttt
tgccccagatggtattctacattcaccttctgcaacaagacggtacatttttggcagtattacagttttaatgtttatggaatgaagatgaaacattaac
gcaaatcaaggcagaagagggacacatacgtaattgtacaattcctgatttatttttcagctgtttaactagaaactgtcattatgctgtaatttttgga
ggggctattccggccaattctaagtgcatccttgcagcttgcatggttttcaataggcttgccccctgctgcggctccttcatacaatacacagatggac
ccatcctcaccaatgcgataggagacttcaaaagggtcaacccaaagagtcagctcgctggggagaagctggaacagtctctggtggctgagcccaatgt
gacctgctgcctgtcctatcaaggggtccattttgtggttaattctcaagcaacggtaccctgatcctttgaaagggttctgcggaaaccagtggtgttt
ataatgatccttcatggcctcctgtaggacctggcagaagtgctgcagctgcctctcctctatgaccccggttgttttcagcaaacttgtgataaacgca
gcagctgttgagatctcagtcatcattgtggcatataaggttgaatcctcacagctatataaacatcaacagcagcgggtctctctcttcgatcgtatag
gcaggacagaatcgtaagcgat
3' EST: DT529551 [+]tatagggcgagaggtgacatcggacggagtgagagggctagctctggcctgcttcccccccttcagcgaaacactcgggaaaatcgggggtagaaagttg
aggactctggctgccggagatcacaaaggaatcgcttacgattctgtcctgcctatacgatcgaagagagagacccgctgctgttgatgtttatatagct
gtgaggattcaaccttatatgccacaatgatgactgagatctcaacagctgctgcgtttatcacaagtttgctgaaaacaaccggggtcatagaggagag
gcagctgcagcacttctgccaggtcctacaggaggccatgaaggatcattataaacaccactggtttccgcagaaccctttcaaaggatcagggtaccgt
tgcttgagaattaaccacaaaatggaccccttgataggacaggcagcaggtcacattgggctcagccaccagagactgttccagcttctccccagcgagc
tgactctttgggttgacccttttgaagtctcctatcgcattggtgaggatgggtccatctgtgtattgtatgaaggagccgcagcagggggcaagcctat
tgaaaaccatgcaagctgcaaggatgcacttagaattggccggaatagcccctccaaaaattacagcataatgacagtttctagttaaacagctgaaaaa
taaatcaggaattgtacaattacgtatgtgtccctcttctgccttgatttgcgttaatgtttcatcttcattccataaacattaaaactgtaatactgcc
aaaaatgtaccgtcttgttgcagaaagtgaatgtagaataccatctggggcaaaaagctcttattgncatttctgtctttctttatgacattttctcgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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