XB-CLONE-3061240
Clone Name: IMAGE:7846414
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Gene:
srl
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT529746 [+]
agccaattatgatttcactttattctaggtttctaactccaccataaaaaaaagaacaataacaaaatagctctataatgttctgtttggagaccacggt
gacgggggcaaatgagaaatctcaatttactgtagtctcttctgatacttctggcattgcaaaaacccaaataggaggaattttttaacgatgacatttt
ctaatgttatgatggtacctccatcaatgtaaatatgtaaactccatggctaaacctatgttctgtggaacacaaagtcttccctgtagcaaccagtgag
ctggtaggttttaattatttatgatatcgacatgagggcttctgttttatggatctactgttaactacctatttagacaatataattaccaatcagggtg
ggaagcgatgctcagtttaccatacctgtactgagtttatctgatgtatgtgttgctatgccatggctccatttgaacggcctatatttaaaacaacatt
actgtctgtgtgcatattttttcttagtttcttgctaaaccgtaagtctgcttgctgcatcagaatttgttgtttttcttatttgtcacattttttttta
ccgttttacaccagttgctgttctgttccttagcaacgtctaatctcacagaagctgaagaagcaatgggcagaaatggctttttttcttatctttacaa
ggccatctatgcatgtaacaacgcaccagtagtatcagcagcttgaggttacaataatagatttttgtatgcacaaatgccagacacataaagatgcgtg
gtctctgttcgcgtgattacaatttacccacatgtgatta
3' EST: DT529745 [+]acaagctcaaactattttattgtatttcaagaggagaatcagtccagaggctcataataacaaactggaaggacagttgatgataaaaaaaaaaaagata
ttaaatatttggaccaagaaagtgtcatctgctgcttaatctattcatttgtatctaactctactttatattaatccaacaagagctgaaggggcctgca
ggacaataatccatgcttccatgatggttttcaataacctgatcaaaatcttccagaaggaatatctcaattatctccagatgcatctcactattgcgat
cacccctgcaacagacgcgggggcccttcagcacgcaagcgattaactaaacttacaaaaaaatcacactgaagtcaaaacacttagacctttaaccctt
ttacgctaaccttccagtctcccactcatatatctgatgtataataatatcctgattgatttcctactcacatttaaagggttaatgaggctttttttct
tactgaaaccctttgtattatattgtttagtggatattagtgaactatattatagcagcattactgtctggtgtatatatatatatatacttcatttaat
ttacacttaatgacagtttcttaaaaaaaaaagtaacttccgtaatcacattgtggtaaattgtaatcacgcgaacagagaccacgcatctttatgtgtc
tggcatttgtgcatacaaaaatctattattgtaacctcaagctgctgatactactggtgcgttgttacatgcatagatggccttgtaaagataagaaaaa
aagccatttctgcccattgcttcttcagcttctgtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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