XB-CLONE-3061237
Clone Name: IMAGE:7846462
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Gene:
slc4a7
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT529748 [+]
attttacagatcctaaaatacccaataacgtacttgcaaagttctttttatcgtgcagtttttggattggaaaactgagtagaaaatggggcactattgt
agtttagcaatatattttcaaaaaagtgattctcattaacagtattgcataacttttacataatttcattgtatttgtacaattttggtgttgtggaaaa
acattcttaagatattagaagttagtggaattttttagttcttatatgtttgaaaaagtaagtgatcctttactgtgactctaatctgtcgaagtagaac
tctttgcgtcggtataaagtagtgcattcaacttatttatttataaggctgatgtcccagagtgtgttagttgcgcacgactgcaggcaactttgcaaaa
ccgaagggaaggcttttccactggcgactcccacgatatcggagatatgacgcaggtgactaacacattccgtgggacatcagtctatagctgttgtatg
ataaaagctaagctttctgttgtatgtaaattccagcagttaagggttctgggggcactgattctaaaagtatttttgctaaaagtatgctcttaatttg
aagtgtatggaaactggtagtcgcatacaaacttgcatagaacaaatttactagcgctgctttcagtttattttggatccaagtgtattaaataggcagt
gttttacacagtgattggctgtgtatttctgtgccacttcttacacatttataaacatgctccttaggttgttaaggctaatgtcaatccttttctcacc
agcagagaaatccttttaggggcaaaatttacctgtggcagtctttgatcatgactttagcct
3' EST: DT529747 [+]ttttttacttctcctacagaagttacacgttttgaaaaactgatgaggaggtttggcatccctgttaaacagcacatattacaaaattagaaaagtgcct
tgcaggctgttgtggcatgtataatcagaccatatacaaataagagaactgggagattaggctggaaagtttgttgccaaacctttaagactgctcagtt
taaggctaaagtcatgatcaaagactgccacaggtaaattttgcccctaaaaggatttctctgctggtgagaaaaggattgacattagccttaacaacct
aaggagcatgtttataaatgtgtaagaagtggcacagaaatacacagccaatcactgtgtaaaacactgcctatttaatacacttggatccaaaataaac
tgaaagcagcgctagtaaatttgttctatgcaagtttgtatgcgactaccagtttccatacacttcaaattaagagcatacttttagcaaaaatactttt
agaatcagtgcccccagaacccttaactgctggaatttacatacaacagaaagcttagcttttatcatacaacagctatagactgatgtcccacggaatg
tgttagtcacctgcgtcatatctccgatatcgtgggagtcgccagtggaaaagccttcccttcggttttgcaaagttgcctgcagtcgtgcgcaactaac
acactctgggacatcagccttataaataaataagttgaatgcactactttataccgacgcanagagttctacttcgacagattagagtcacagtaaagga
tcacttactttttcaaacatataagaactaaaaaattccactaacttctaatatct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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