XB-CLONE-3060873
Clone Name: IMAGE:7846687
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Gene:
psmb7
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT529989 [+]
ccgagggttttttttaatggatcataagataacgcagaaaatgcttaacatgataatgtgggctaacacgggatgcaaaaagatgccaatacatttactt
aagagacttaggaaatatccatagtttgcacggattcttccatcacatccaagttgaagttggtaatcttctccgacagaatccccgtggtacctttctt
gtatttataatttcctgtcctcacgcctttcttgttggccaactcgtggggacggatataatccaccttattttttgttattacgcatagatcaatgttg
ctgccggagcccaggtcattaaagatcccagcggcgatggcatcgcgtaccagctgcttggcttcttcttcctccatgtcaggtttgtagcgatcctcaa
aaacagccatggcagccagcgaaccagagcccatagtaacgtagggaagcttgtcagtggatccatgagggtaaatgctgtacagatggggcccagtaca
gtccactcacccagtaccagcgcagcaccaatataaccttgatacctaaatagcatctgcttcagcattcggttggcagttgctaccctggataaacgtc
ccgtggacagagaatgtagctccatgttggaagagatcatctgcgttgtcatttctgtatcagctgcagtcccagcaccacagcaatagacatttggagc
tatatagtggatctttgagcagttcttatcagcaacaatcattccttctgttgctcttgtgtcagctccaagtataactccatccttaaacactattcca
gcaattgtggtccctgttttgcgagctgcgggtagcttgtgccctttctgagtaaattcattggtcaagagggtatttctcctgcagttgctgaaatcaa
agctctcgc
3' EST: DT529988 [+]ttgatttcagcaactgcaggagaaataccctcttgaccaatgaatttactcagaaagggcacaagctacccgcagctcgcaaaacagggaccacaattgc
tggaatagtgtttaaggatggagttatacttggagctgacacaagagcaacagaaggaatgattgttgctgataagaactgctcaaagatccactatata
gctccaaatgtctattgctgtggtgctgggactgcagctgatacagaaatgacaacgcagatgatctcttccaacatggagctacattctctgtccacgg
gacgtttatccagggtagcaactgccaaccgaatgctgaagcagatgctatttaggtatcaaggttatattggtgctgcgctggtactgggtgagtggac
tgtactgggccccatctgtacagcatttaccctcatggatccactgacaagcttccctacgttactatgggctctggttcgctggctgccatggctgttt
ttgaggatcgctacaaacctgacatggaggaagaagaagccaagcagctggtacgcgatgccatcgccgctgggatctttaatgacctgggctccggcag
caacattgatctatgcgtaataacaaaaaataaggtggattatatccgtccccacgagttggccaacaagaaaggcgtgaggacaggaaattataaatac
aagaaaggtaccacggggattctgtcggagaagattaccaacttcaacttggatgtgatggaagaatccgtgcaaactatggatatttcctaagtctctt
aagtaaatgtattggcatctttttgcatcccgtgttagcccacattatcatgttaagcattttctgcgttaatctatgatccattaaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: