XB-CLONE-3060729
Clone Name: IMAGE:7846605
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Gene:
tuba4b
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530084 [+]
ggaatgcatctccgtcccgtggggcaggcaggagtccagatgggcaatgcatgctgggagctgtactgcctggaacatgggatccaaccggacggccaga
tgcccagcgacaaaaccattggtggaggggacgattcttttaccaccttcttcagtgagaccggagccgggaagcacgtcccccgcgctgtgtttgtgga
cctggagcccactgtgattgatgaggtgcgggcaggcacataccggcagctcttccatccagagcaactcatcacaggcaaggaagatgccgccaataac
tacgcccgggggcattacaccatcggcaaggagatcattgacccagttctggacagaatccgcaagctggcggatcagtgcacgggactgcagggctttt
tagtcttccacagcttcggggggggcaccggctctggattcacctccctgctgatggaacgtctctctgttgactatggcaagaagtccaagctggaatt
ctccatctatccagctcctcaggtttcaacggctgtggtggaaccctacaactccatcctcaccacccacacaacactggagcactcagactgtgccttc
atggtggataatgaagccatttatgacatctgcagaaggaacctggacattgaacgcccaacctacaccaacctgaaccgccttataagccagattgtgt
cctctatcacagcctccctcagatttgatggagcactgaatgtggacctgacagaattccaaaccaacttggtaccctacccccgtatccatttccctct
ggccacctatgcccctgttatctctgcagagaaagcttaccatgagcagctctctgt
3' EST: DT530083 [+]gaatttttttcactaaaataacctcagctgctaaaaataagatattcacattaaaaataaaatccgagaggaagctggcaggatatacttttctagcatt
gggcttttacaggttgggaggggcagtgttaaactaggccaactggcaccttccaatctttggaataataagatgatggataaagggcaattaatactcc
tctccctcatcttcatcttcataggagtcaatgccgacctcttcgtaatccttctccagggcggccatatcctcgcgggcctcagagaactccccttcct
ccattccctcccctacataccagtgcacaaaggcacgcttggcatacataaggtcaaacttgtgatccaggcgagcccaggcctcagcaatggcggtggt
gttgctcaacatgcacacagctcgctgtaccttggccagatccccacccggaaccgctgttgggggctggtagttgataccaaccttgaaaccagttggg
caccagtccacaaactggatggtacgtttggtcttgatagcagcaatagcagcattgacatctttgggcaccacatcaccgcggtacagcaggcaacaag
ccatgtatttaccatgacgggggtcacatttcaccatctgattggctggctcaaagcaagcattggtgatctcggatacagagagctgctcatggtaagc
tttctctgcagagataacaggggcataggtggccagagggaaatggatacgggggtagggtaccaagttggtttggaattctgtcaggtccacattcagt
gctccatcaaatctgagggaggctgtgat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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