XB-CLONE-3060637
Clone Name: IMAGE:7846709
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Gene:
chmp3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530144 [+]
aatggtcactcaaaatccggaaagaaatgcgagtgattgacagacaaataagagatatacagagggaacaggagaaagtcaagcggtcaatcaaagaatc
ggcaaaaaaaggcaacagggaggcctgtgtgatattagccaaagaggtggtgcattcaaagaaagccgtcaacaagctgtatgcatccaaggctcacatg
aactccgtgctaatgagcatgaagaaccaactggctgttcttagagtctctgggtcattgcagaagagcacagaagtcatgaaggccatgcaaaacctag
tgaaaatacccgagatccaggctaccatgagagacctgtccaaagaaatgatgaaggcaggtataatagaagaaatgctggaggacacatttgaaggcat
ggaagaccaggatgagatggaggaacaggcagagatggaaatcgataggatcttgtttgaaatcacggcaggggccttggggaaagctcccagcaaagtc
acagatgccttaccggagcctgagattacgggggccatggctgcatccgatgaagaggaggaggaggacctggaggccatgcagtcccggctggcagccc
tcaggagttaaggacgtactgtacatattcttattctaggagacttccctggtttttgaaatgtaaactttcttttgtgaaaatattttggtttatatat
atatatatctctatacagctgtggcctaacctttcctccaaccatattgatctctctctgctttacaaccaatgtccctgggcccatctgctattcctat
tgngtatggcatgtttttgcactgtgctcctctttgctactttaagccccagcagcactttataaaccaggcaaatccccatctgttatcttcagccac
3' EST: DT530143 [+]ggagtaaaacagcctttaataaatgtacagggtcgcgctgtttccattccatagaaaaatacagtcattcttacacatttacagactctcacccatggcc
ctacggtcaggttcagcacagttactacctgatacagagctcagctggctgctacctcactaaggctatcttgcccagaaattcacctcaacaaacccgg
aatatgacaatacgatattttatctcatacaggaaattcatagtgttctgtaatgcttcaagtgcattgagacagagataaaggaaacgatagcagcgtt
gctctgctgcacttaaagtggaaacttggcaccggtgggggggggggggcattgcctgcagccggaggttcctgggcaactgccattagcgttggttccc
caacacaaaatatgtaacattgttttctgctaaataaagcataaagaaaacagagcaggcgcaaactttccctcaggggtttactctgagccaattcaga
tccaaacaaaggaaaaatcataaatattgttttggaaaagggaaaactaacctgttgctgaactgtacctcccagcattctcggatagacaaaaggtgtg
tagttctcttccggttctaaacaggctgctatactacaacttggctgtcaaaaaatgctgggagttgtggctgaaaataacaaatggggatttgcctggt
ttataaagggctgctggggcttaaagtagcaaagaggagcacagtgcaaaaacatgccatacccaataggaatagcagatgggcccagggacattggttg
taaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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