XB-CLONE-3060588
Clone Name: IMAGE:7846905
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Gene:
trmo
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123972 [+]
CTTATTTCAGCTTAGACTCAGACGAGAGAGGATTTCTCTGCTGCTCTGATATAAGCACGTTTTTAGGAGGCAGAACAGCAAATACAACGTAATGTGCCAT
CACCAAAGCAGAACGAAACTATTTTCAACCTGAGCAACCTGCAAGATCACTTTTAGAGAGAGAGTCCCCCAAATCAGCTTTTTGTCTGGAGAACGTGGCG
GTGTTGCTGTCAAGAGATAAGTAAAGAGCAGAACTTGACAGGAGTTAATAATGCAGTGTGATGACCGAAATAAAAAGAAAAAAGAAGTGCAACCCGAGAT
TGGGTCTAACTTCCTGGAGGCGGGAACCATATTAACAACCCCAATTGGATACATTGAATCCTGCTTCATGACAAAGAATGGCACTCCCCGGCAGCCATCA
GTTTGCAGTTTGTCACGTGGCCGTTTAAGAATAAGCAAAAGTGTCTTCAACAACCCTGAACATTCACTAATTGGGATAGAGCAATTCTCGCATGTCTGGT
AAGCAAAATGTTTAAGAATGTTACACAGGGTATTTTTATGATGCCATAAATGGAGGCCAAGAGCACATGCCAGGACCAGATCAACTTTAACTGCCTATGC
TCTTGGTTGTTAATCACTGCCAGACAGACATGGTGGCAATGAGCCCCACTAGCCTACTCTGCTATTTTTGTAAACAGGTGCAGGAAAGCAGGGTTGTATC
TAAGTGTGGGTAGTGCAGTTTGGAAAGGAAGAATCCCTTTTTTCTGCCCTAGTGCACCTGCATTTGGCACTTACATGCACCTGTGTTATTACAGCCCCAT
AAGAAAGAATGGAATGCATTTTTTGCTGTGCTCCCCTGCGGGTGGAATGTAGGGGAGCACAGGAAGAAATGCTTGTGAGTAAAAGCCATAAAGCAGTATT
TTGATGTGTTTGTCCGAAAAAGAGTTTTTGTGTATGTATGTAGATAGACTGATCTGAGTGCTCAAGCAGACAGATTGTGAGTCCCTAGTGTTAATTCTAA
TGGAAGTGGGGAAAATTGGGCATAGCAGAGGATTAAAGGGTGGTTCACATTTAAGTTAACTTTTAGTATGTTACAGAATGGCCAATTCTACGCAACTTTT
CAGTTAGTCTACATTATTTATTATTAGTTTTTTTATTATTTGCCATCTTTTTTTAAAAATCTTTGCAGCTTTTAAACAGGGGCCACTAACCCATGCAGTG
GAAAAAAACTATTGCTCTGTGAGGCTACAGTTTAATTGCTATTTTTACTATATATTACTTATCTTTCCATTTAGGTCCTCTCCTATTCATATAGTCTTTC
ATTCCAACCACAGCTAAGGTCATTTGGATCATAGCAACCAAATAACTGCTGGAGAGCTACTGAACAAAAACTGAAATAATTAAAAAATTACAAATAATAA
GGAATGTAGACCAACTGCAAATTGTCTCAGAATATCACTCTCTACATCATACTCAAAGGTGAACAACCCCTTTAAGAAAGGGTGAAAATTGGCTATTTGA
AAACATAAAGCAATAGAAAAAAACAATTATGTTTTCTAATAAACAAGTATGGCTGAAAAAAGGTAGTCGGCACAAAGTTGTCAGTACAGAAAGTGATAGC
ATAAATATTTTTCCTCACAACTCCAACTCCTCACAACCAATTATATTTTCCTTGAATGAATGCTGCTGCCATGTTATACACCAAGATCTTATGCTTACTG
ATTTCTTGCATTACAACACTGGTCTGAAGTTGATTTGGATCTAGTTGTCATGCAAAGTAGTATGTAGTCCCCTAGAAAATGCCTTTTTAAAGAATGACTA
TAGTTTATTTATTTATTTTACCTATTAATGTGTTTTATATAAATACATAAATGTATAGTTATTTGCTTTGTGCCATTACCCCCAGCTTCTATTAATATCA
TCAGGGACAAGTTAAGAAGGGCCTTAAATGGAAGTCCTTCTAGTGGTGACCACACCCTTGGGTTTTGGTTTGTACAAAGAAATAAAGAAAATCTCTGCAT
TAAAATAATTTCTTATTTTTAGCTTGCTCACTTGTGTTTCAAAATAAGCATTTCTAGTATTTATTTTACTGCATTTTTGTAAAATAAAAATAGTCTGCTG
AGGACTTCTAAGAAAACAAAGAAAGCGTTTTTTAAAAACAAGAAAGATAATTATTTTGACAGTAAAGTTGCAAATACTTGGTTCTCAAAATAGTTACCCC
TTATACAATCTTTTGCTCTCTTTTCAGGATTTTGTTTGTTTTTCATAAAAATGGTCGGTTGAGTTGCAAAGCTAAAGTCCAACCTCCAAGACTGAATGGC
GCAAAAACTGGTGTGTTTTCCACAAGGAGTCCACATCGCCCTAATGCCATAGGGTTAACTCTAGTAAGACTTGAAAAGGTTGAAGGTATATTTTTTTTTT
AGATAAACAAGCATATATATTAATTCTTGCTGTTTTACTTTAGATGGTTCCCTTTAACAGTGAATAGACAATTAGAGGCCTGTTGTACTTTTTCATTTTC
CATTTCCATTTGTTGAAAATCAGAAGAGCAGATAAAAGTATTCCTGATTCAGCACTTTTATAAGCTCTTATACTTTTATTTCTGTGTTTTTTTTTACAGG
TTTAAAAAAAAAAAAAAAAAA
5' EST: DT530176 [+]cttatttcagcttagactcagacgagagaggatttctctgctgctctgatataagcacgtttttaggaggcagaacagcaaatacaacgtaatgtgccat
caccaaagcagaacgaaactattttcaacctgagcaacctgcaagatcacttttagagagagagtcccccaaatcagctttttgtctggagaacgtggcg
gtgttgctgtcaagagataagtaaagagcagaacttgacaggagttaataatgcagtgtgatgaccgaaataaaaagaaaaaagaagtgcaacccgagat
tgggtctaacttcctggaggcgggaaccatattaacaaccccaattggatacattgaatcctgcttcatgacaaagaatggcactccccggcagccatca
gtttgcagtttgtcacgtggccgtttaagaataagcaaaagtgtcttcaacaaccctgaacattcactaattgggatagagcaattctcgcatgtctggt
aagcaaaatgtttaagaatgttacacagggtatttttatgatgccataaatggaggccaagagcacatgccaggaccagatcaactttaactgcctatgc
tcttggttgttaatcactgccagacagacatggtggcaatgagccccactagcctactctgctatttttgtaaacaggtgcaggaaagcagggttgtatc
taagtgtgggtagtgcagtttggaaaggaagaatcccttttttctgccctagtgcacctgcatttggcacttacatgcacctgtgttattacagccccat
aa
3' EST: DT530175 [+]aaacctgtaaaaaaaaacacagaaataaaagtataagagcttataaaagtgctgaatcaggaatacttttatctgctcttctgattttcaacaaatggaa
atggaaaatgaaaaagtacaacaggcctctaattgtctattcactgttaaagggaaccatctaaagtaaaacagcaagaattaatatatatgcttgttta
tctaaaaaaaaaatataccttcaaccttttcaagtcttactagagttaaccctatggcattagggcgatgtggactccttgtggaaaacacaccagtttt
tgcgccattcagtcttggaggttggactttagctttgcaactcaaccgaccatttttatgaaaaacaaacaaaatcctgaaaagagagcaaaagattgta
taaggggtaactattttgagaaccaagtatttgcaactttactgtcaaaataattatctttcttgtttttaaaaaacgctttctttgttttcttagaagt
cctcagcagactatttttattttacaaaaatgcagtaaaataaatactagaaatgcttattttgaaacacaagtgagcaagctaaaaataagaaattatt
ttaatgcagagattttctttatttctttgtacaaaccaaaacccaagggtgtggtcaccactagaaggacttccatttaaggcccttcttaacttgtccc
tgatgatattaatagaagctgggggtaatggcacaaagcaaataactatacatttatgtatttatataaaacacattaataggtaaaataaataaataaa
ctatagtcattctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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