XB-CLONE-3060585
Clone Name: IMAGE:7846571
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Gene:
psma6
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530178 [+]
tcagcgcggggtttgacaggcacattaccatcttctcacccgaagggcgtctctatcaagtcgaatatgcattcaaggcgatcaaccaggggggtcttac
atcagtggctgtcagaggaaaagactgtgctgttgttgttacccaaagaaaagtcccagacaagctgttggacagcaacacagtaacccatttattccgg
attactgaatccattggctgtgtaatgaccggaatgacagctgacagcaggtcgcaggtccagcgggcacgttatgaggctgcaaattggaagtacaaat
acggctatgaaatcccagtagatatgctttgtaaaaggattgcggatatttctcaagtatacacacagaatgctgaaatgaggcccttaggatgttgcat
gatcctaattggagtggatgaggagaatggaccacaagtctttaaatgtgatcctgcaggctactattgtggctttaaggctaccacagctggagtgaag
caaactgaggccaccagcttcctagaaaagaaagtaaagaagaaattggactggacttatgagcagactcttgagacagccatttcttgtctctccacgg
tactgtctattgatttcaaaccttcagaactggaggtgggagtcgtcacagtcgaggatccaaaattcagagttcttacagaagcagagattgatgtcca
ccttgttgcactagctgagcgtgactagtcatcctactttcacctctgcccagaaagctcttgcctggtaaaatcacctgtcttcataacactttttgtt
ttgttctctacataaataaaacagatttttttctttttggaaaaaaaaaaaaaaaaactcgagcctctcg
3' EST: DT530177 [+]ccaaaagaaaaaaatctggttttatttatgtagagaacaaaacaaaaagtgttatgaagacaggtgattttaccaggcaagagctttctgggcagaggtg
aaagtaggatgactagtcacgctcagctagtgcaacaaggtggacatcaatctctgcttctgtaagaactctgaattttggatcctcgactgtgacgact
cccacctccagttctgaaggtttgaaatcaatagacagtaccgtggagagacaagaaatggctgtctcaagagtctgctcataagtccagtccaatttct
tctttactttcttttctaggaagctggtggcctcagtttgcttcactccagctgtggtagccttaaagccacaatagtagcctgcaggatcacatttaaa
gacttgtggtccattctcctcatccactccaattaggatcatgcaacatcctaagggcctcatttcagcattctgtgtgtatacttgagaaatatccgca
atccttttacaaagcatatctactgggatttcatagccgtatttgtacttccaatttgcagcctcataacgtgcccgctggacctgcgacctgctgtcag
ctgtcattccggtcattacacagccaatggattcagtaatccggaataaatgggttactgtgttgctgtccaacagcttgtctgggacttttctttgggt
aacaacaacagcacagtcttttcctctgacagccactgatgtaagacccccctggttgatcgccttgaatgcatattcgacttgatagagacgcccttcg
ggtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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