XB-CLONE-3060410
Clone Name: IMAGE:7846540
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Gene:
mypn
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530292 [+]
ggatacccatacccaaggtttattggtttaaagatggcaagcagatatcaaagaaagatcaacactgcaaaatgcagagggaagcagatggaacatgcgc
tttatatattgaagctgcaacgaacgatgatgatgggaactacacaattatggcagcaaatccacagggaagaatcagttgctctggtcatttgctggta
cagaatgctccattccggcctcggtctcttctccctgttcagtctcacagggcgaggccacgtgttccagaagtagagagcgaggtagtagaggagcgat
tcttccggccatactttcttcaggctccaggggatatggttgcacacgaaggacggctctgccgacttgattgtaaggttagtggacttccaactccaga
tatcatgtggctactcaatggaaggcaagttaaaccagatgccacccacaaaatgcttgtcagggagaatggaattcattcgctccttattgatcctgtc
acaaaagcagattctgggacctacatttgcattgcaactaataaaactgggcaaaactcattcagcctggagctcagtatcatggcaaaagaagtccaaa
aagttccagtatttttggaaaaacttcaaaattgtggtgtcccagaaggtcatccagtcagattagagagtcgagttgttggaatgcccccaccagtgtt
ctactggaagaaagacaatgaatccattccggccaataaaccacggataagcatgcatcaagataacactggttacgtgtgcctcctcatccagcctgcc
agaaaagaagatgcaggatggtacacactgtcagcaaagaacgaagcagggat
3' EST: DT530291 [+]gcaacatgttgtaactttattagggaatacatcccattttcacttcctataaacaaaccgccatttgttaaatagcaagagatggcacttttagtttaaa
ccctctactgtataagcaaccttgttttaacaaagaatctggtaaaatacaaataatgcacatttatatattaatataacctctataggtctttactttc
ttttctttgtggacataaatatttaaatagggtacttatacaacatcaaacagatcacactgcatcagtgtcagtgccacgggcatacagatcactgagt
actgctggcgcgtctgggctacatatactgcaaacctatgttaacctgttcactgccagaaagggctgggcccctctagtggcactgaaacagtactacc
tggcagttagattattttatattggcaaaacactgaagccaaacagggatctttatccccattaaaaatttgatttgtaaacgttcaagctgtattttct
tcacagttttattttgggttttgacgtcggttccattcacagctcttcactttctaccacttcccgtgtagacaatgagtaattcagcggcgttactaca
ggaggatgagagctgatggcatccaaagctgaaaacatagaataccgagaacctgttgatcgggtctttttgattggctggggaatctgctggtaccact
gagcataaatatcaagtcttgcagtacaggatacaatccctgcttcgttctttgctgacagtgtgtaccatcctgcatcttcttttctggcaggctggat
gaggaggcacacgtaaccagtgttatcttgatgcatgcttatccgtggttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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