XB-CLONE-3060247
Clone Name: IMAGE:7846602
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Gene:
arap3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530400 [+]
ccgaggcaaagtttacatgggcattaggaaaaagatgaaggctccatcccaatggggttttaccatctacttggataaacagcagctgtgcctgtgttgt
tcaggtcaaccagaaatgtgggactggataaccagtattctgaaggctcagcatgacgatctccgaccggtgattttaaggcgccactcatcctccgatg
tgactaaacaaaagtttggcacaatgccattggttccaatccgtggagatgacaccaattctactatggttactgcaaatcagacactgcgccgacttca
tacaagaaggaccctatcaatgttctttccaatgaaaatgcatcaagattcacttgaggaactgcaggaaaaacaaagtgaagaatatgatgaggctgta
tatgaggaagtgggaaattttcagagtctaccacagtcagagttagaatttgcatcagaaaaacaacttgttcctccacctttggatcgaaccaaaaaac
ctgctctcacccaggatctaaaaaaactaacagttcccgcaactttagaccaaagaaagagctgttccccagaaaggccagaaagggacagctacagaaa
atctcactctctagacagaaatgtgtgcgtggaaagttctgtgacaggccatagtgaaaattttaaaccttcatggacagaaagaggcattacttctagc
cttctaaaaactggctctcttgaacgaacagtggaaacaagtcgtctacaggcagtggccacaagaagccagggggcaaagagcacattggacttaagtt
ctctgcctccgttgttttttgagccagttcctccatcagacacagcaaagaatccagcatggaagaaaaatcaggagttcc
3' EST: DT530399 [+]aggatgcattcactatttaatttcaccacccaaactgattgagaaaatgttcacaaatgtgttgtaatgcttgcttttgggtcatacaggaaactttaaa
agctccttcaccttatataaaatataacttaataggcatagatcgataaatagatttaaaacaaagaacacatctatatacaagctgtatgtgtgagagc
agctagagtttggacaagctgcaattcttggtccatttctcaggttcccacagtacaatttgcctaatgttctcttgctaagggcgaacagaacttgaaa
aagaaatgtgttaactacatcacgtgacttgttgccctgttgatgcggcatcatttttcttaagaatgatagcactgagttcctggacaagtttatcttg
tatttggttgggaactcctgaatttttcttccatgctggattctttgctgtgtctgatggaggaactggctcaaaaaacaacggaggcagagaacttaag
tccaatgtgctctttgccccctggcttcttgtggccactgcctgtagacgacttgtttccactgttcgttcaagagagccagtttttagaaggctagaag
taatgcctctttctgtccatgaaggtttaaaattttcactatggcctgtcacagaactttccacgcacacatttctgtctagagagtgagattttctgta
gctgtccctttctggcctttctggggaacagctctttctttggtctaaagttgcgggaactgttagtttttttagatcctgggtgagagcaggttttttg
gttcgatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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