XB-CLONE-3060061
Clone Name: IMAGE:7846666
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530523 [+]
aatccacgcaacagagaaagatattggaaaatacacaattaaagcaaagaacttccatggacaatgctctgccacagcatccttaaatgtgcatcatctt
atggaagaacctgtcaagcaggttgtattacagacagctggggaagtcagccttcaggaaggattctccttccaggcaatgaaaatgtcttcttccaggc
aagaggcctccttcagcagtatctccgagacaaagagcatgtcctcaaaaagtgaggcatcatttgttgaaatgagttcaagtagctttatggaaaaatc
cagtctgatgcagagttcatctagcagaatgcttacagctggtttcagaggaatacctcccaaaattgaagctcttcccaatgatgtcagcattgacgaa
ggaaaagtactaactgtggcttgtgccttttctggagaacccacaccagatattgcatggtctaaggctggacatccagttcccactcaagaacaaaaag
gcagagttcacattgaaacttctgaagacttaaccactcttatcatcatggacattcagaagaaagatggcggacattacactcttaacctgtcaaacga
ctttgggtccgattctgcatctgtgaatattaatgtccgaacacattaagcattttatcgtttacattcacatgtaattttacaagtgattcactagaac
tagatgaactgatttgtaaatataaaagctatttttgtacctacctgtataagacaaagtcgaaaagatttacgctatcacatattcctatttgaatagg
gaaattgatacatttttaactgacatgtacatactgtatatagcc
3' EST: DT530522 [+]aatggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataat
gacgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctat
tctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtg
atgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacatacaaa
agttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaatatt
cacagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgct
gaggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttcc
agtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatcaat
ttccctattcaaataggaatatgtgatagcgtaaatctttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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