XB-CLONE-3060034
Clone Name: IMAGE:7846764
|
Gene:
eef1a1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT530541 [+]
ccgaggacaatccacaatgggaaaggaaaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaaccggacatcttatc
tacaaatgtggtggtatcgacaagagaaccatcgaaaagtttgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggaca
aactgaaggccgagcgtgaacgtggtatcaccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggaca
cagagacttcatcaagaacatgatcactggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctca
aagaacggacaaactcgtgagcatgccctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccat
acagccagaaaagatatgaggaaatcgttaaggaagtcagcacatacatcaagaagattggttacaaccctgacactgttgcctttgtacctatttctgg
atggaacggtgacaacatgcttgagcccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactaccctg
ctggaagctcttgactgcattttgccaccaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggtactg
taccagtggntcgtgtggagactggtgtccttaagccaggcatggtggttacttttgcccctgttatgtaacaactgaagttaaatctgttgaa
3' EST: DT530540 [+]cctatagggcgagaggcttttaatggtctcatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaacactaataacttaa
aaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgat
ggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctg
agcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacga
ccaagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgt
cttccagtttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatcc
agcaccaatctggcctgggtggttcaggatgataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgcca
cgacggacgtccttcacagaaacgtttttcacgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaa
cttcagttgttacattaacaggggcaaaagtaaccaccatgcctggcttaaggacaccagtctccacacgnacaactggtacagtaccaataccgcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: