XB-CLONE-3059929
Clone Name: IMAGE:7846678
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Gene:
snta1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530611 [+]
ctgtcccggggagcgctgggtgcgggtactgctgctgctcggagaggagactgtgactttcaccccggagccggagcccggggcagcggaggagaaggcg
ctgaacggcgcggagccggcggggggggtcccggagtctctcattaacctgcgccggactgtcagggtgctcaagcaggatgcgggggggctgggggtca
gcatcaagggtggccgggagaacaagatgcccatcctcatctccaagattttcaaaggcctggctgctgacttgacagggtcattatatgtgggggatgc
aatcctgtctgttaatggggtagacctgtcagaggctactcatgatgacgctgtgcaggctctgaagaagactggaaaggaggtggtgctggaagtaaag
tacatgaaggaaatctcaccgtatttcaagggctctcccccgagtggttcacctgctgcccctttacagaagagggggtcccccgcgctcccacccaagg
aatacaaagatggaaagagcattccgctgaagatgtgctacgtatcccggagattcctaccaacggacaccgagcccaggtacctcgagatctgctcagc
agacggaagggatgtgctgttctttcgggcaaaggatgaaggtacagcccagtcctggtttaatgccatccacaccaatgccagcgccctggcgccccgt
gtgcgggaggatgtgaagcagctattgggcaaagacatcaaacagatgggctggctcactgagcagctcccccaggatggaaagcgcaatctgctggccc
tactcacagagaaggatctcctgctgtacaacagcctcccgcactccagggaggggctca
3' EST: DT530610 [+]actcagacactttattgtttattgtacacctccaaagggcaagtaaacacnnctccgtttctttccaaacaatcaggatcatgaaacgtcctcccatata
tatatatatctatatccacacgctaaatactttgtaaaataataggttcaagtccagaataaacccccccaaatgcccttattctaatgcagcttaaaaa
gttgtgccgggtagcttaatttcaataattaataaaactagatacatatctgcctctatgatcatttatgaatatatattaatatatttatacaaacgcc
ctaacctgtgctccgaaggggaaacaattagaccagattgatagctgattgtagagcgggttgtgctgctttataacaacatggcgtggagcagatattg
ggcatttaaaggcacagtacttttgtttttccccaaaaacccactgtatatcagagagactgacctggattctccctgaagccatggcattaaacctttg
aaaaagaaatatatatatatataaggcactataattcatggttagggaatcggcagtttacaaagaataaagacgagaggcttcacatttccttgccatt
aaccgtatccagacatagtgacatagaagtggttaattctggccatttatggtgtgtaaatacaaatattatttgtgtgacattttggaaagctgtccac
tttggactaactctccagcaccccctagggttggttttggttagcgctcggccgcgaggtccaagattgccgccactttcagcgttctaggtgaataggc
ccagtcgggacactttggccgacaggaaggagtgaatgatgaagacaatggtttttggacaggagtgcaggtcgagttga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: