XB-CLONE-3059755
Clone Name: IMAGE:7847029
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Gene:
lsm12
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530726 [+]
tgccggacctgccaagagacgagactgcagggcgaggtggttgcctttgactacccaagcaaaatgctggcgctcaagtgcccctcctccagtgggaagc
ccaaccatgctgatatccttctcctgaatttggactatgtgtctgacgtagaggtgataaacgaaagaacacagaccccaccgcccttagcctctctcaa
tatcaccaagcttgccagcagagctcgcttagagaaggaagagaagttgagccaggcctacgctatcagtgctggagtgtcactggatggacagcaactt
tttcagaccattcataagacgataaaagactgtaaatggcaggagaagaatatagtagttatggaagaagttgtaatttctcccccctaccaggttgaaa
attgtaaaggaaaggagggccgtgccctgtgccacgtctgcaaaatagttgagaaacactttatagatatggaaaatcaaaagggggtgcagagaaatcc
agcccaacagtcacagaaagagaccagcacgtcatcctgagccttcgaatcatgttggcctcgccttccccatgctgcctgctcttctttccaccagtag
tgtggttgtccaaaggagagctatagggggtggaccacatgggggtgggtgagcggggatttgggtgggattgggtagcttttatttctggactagtcac
tcaaccctttacttttttaaaagccattattctcagtcttggcttcaggaaggggttgaggacatactatgttctatggaagaccccaagggcttcattc
agtcatccagaatatttttttctttagccatttgggctctaattcagaggttttttttccccaacattgcatttcttccacagcagattttcccctcccc
tttatt
3' EST: DT530725 [+]ttttgtacaggcagttttataaccgctgcacagccagcgagggacatgccttgcattgccattcccctctggctgagtatggttgcactttcccccagca
acagacagaaaaaaaaaatctttacacaaataaaggggaggggaaaatctgctgtggaagaaatgcaatgttggggaaaaaaaacctctgaattagagcc
caaatggctaaagaaaaaaatattctggatgactgaatgaagcccttggggtcttccatagaacatagtatgtcctcaaccccttcctgaagccaagact
gagaataatggcttttaaaaaagtaaagggttgagtgactagtccagaaataaaagctacccaatcccacccaaatccccgctcacccacccccatgtgg
tccaccccctatagctctcctttggacaaccacactactggtggaaagaagagcaggcagcatggggaaggcgaggccaacatgattcgaaggctcagga
tgacgtgctggtctctttctgtgactgttgggctggatttctctgcacccccttttgattttccatatctataaagtgtttctcaactattttgcagacg
tggcacagggcacggccctcctttcctttacaattttcaacctggtaggggggagaaattacaacttcttccataactactatattcttctcctgccatt
tacagtcttttatcgtcttatgaatggtctgaaaaagttgctgtccatccagtgacactccagcactgatagcgtaggcctggctcaacttctcttcctt
ctctaagcgagctctgctggcaagcttggtgatattgagagaggctaagggcggtggggtctgtgttctttcgtttatcacctct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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