XB-CLONE-3059683
Clone Name: IMAGE:7847083
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Gene:
apcs
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530773 [+]
ctgaatagtaatattncattttttcaattaaacctgctttgcagtgcttatctattgtcagggatgtctgttaaagagaagagctccttgaagatggtga
gatatgtcctgtggtttgtcttcttcaccggatgccttgcacaagaagatttaggaggaaaagtctttctttttcccaaggccactgacacagcccatgt
gattttaaagcctgtgataaaaaagccagtagagagtttcaccgtttgcctggaatcctacacagaattgactcgagaacattcccttttctctttggcc
gttccaggaaaggacaatgctcttctcatcttcccaatgccaccgaccacatgtcggtttattctaaaccaagaacctgtcgatcccaaagtacaaccag
aggtttttgattggaaacatacctgtgtagcttgggaatctgggtctggagtggttcagctctggatcaatggaaaactgtatcccagaactgttttaaa
gaaggggccactgattgatgctaaggccagtataatcctggggcaggaacaggattcatatgggggcggctttgacatcagtcagtcctttatgggcgag
ataagcaatgtgcacatgtgggattatgtcctgacccaggtagacatacagaaggttctggctggaaaaaaggatgtaaatggaaatatcattaactgga
gatcattgcagtatgagataaaaggagatgtgactgtccaacctaagctacaatgtcggtctctacagaaccattataacttgctttcttcatgcaatga
aacttaattacagtattttgcatgatggagtcacgcgccacatatctatactggtttcattct
3' EST: DT530772 [+]ctttatttagttctaaagagcttgttaaaacattgcataatacattgacaaccaaggtgactgtatcatgggaacagtaagggacatctctagaaagcaa
cctacaaaggccggactgattgcagttgatcggaacacgataaccaatagtatttattagaagcctccctgatttttcccacaatcccattaaatagaat
aagctaattatgaacactacagctataaagcatttagatacttagtaaatcagaatgaaaccagtatagatatgtggcgcgtgactccatcatgcaaaat
actgtaattaagtttcattgcatgaagaaagcaagttataatggttctgtagagaccgacattgtagcttaggttggacagtcacatctccttttatctc
atactgcaatgatctccagttaatgatatttccatttacatccttttttccagccagaaccttctgtatgtctacctgggtcaggacataatcccacatg
tgcacattgcttatctcgcccataaaggactgactgatgtcaaagccgcccccatatgaatcctgttcctgccccaggattatactggccttagcatcaa
tcagtggccccttctttaaaacagttctgggatacagttttccattgatccagagctgaaccactccagacccagattcccaagctacacaggtatgttt
ccaatcaaaaacctctggttgtactttgggatcgacaggttcttggtttagaataaaccgacatgtggtcggtggcattgggaagatgagaagagcattg
tcctttcctggaacggccaaagagaaaagggaatgttctcgagtcaattctgtgtagga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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