XB-CLONE-3059608
Clone Name: IMAGE:7847233
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Gene:
ppa2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530823 [+]
cgcattacaatgacatagaagatgtcagacgactgaaacccaattatctggagtccaccgttgactggttccgacggtacaaagttccagatgggaagcc
agagaaccagtttgcctttgacgcagaatttaaaaataaggattttgccattaatataattaaaagtacccatgagcactggaagtcactggtgactaaa
agaatagaaggaggagaaatcaattgcactaacgtcacggtacaagatagccctttcctctccaaggcagaagaagtggaatccattgtgaaggcagcgc
ctccttgtggaccccctagccctgttgcagaagatgtggacaagtggtactattaccagaaaaactaattattgggtgaagacttagagcaattactgca
tgcttttatccagtcgctgggatctgcagtacagagaagactctggcaagaaaataaatatgactacagtaaccaatccatgtcaagtacaaaatgcccc
cttcccctggcacttagctttattatgcaacccattctgccacctcagggtcaccctggatattaacacctttgagcacctgcagcagatctcccttata
atagaaatctacttccaggattacagtaatattatttggaatatttgtgtttttgtgaaagcagattagcaggattaaaatgcagatatctgctaaattg
tttaacctctgtggaggttgccttcatcaatagaataaagctacttttgaggataaaaaaaaaaaaaaaaaa
3' EST: DT530822 [+]atcctcaaaagtagctttattctattgatgaaggcaacctccacagaggttaaacaatttagcagatatctgcattttaatcctgctaatctgctttcac
aaaaacacaaatattccaaataatattactgtaatcctggaagtagatttctattataagggagatctgctgcaggtgctcaaaggtgttaatatccagg
gtgaccctgaggtggcagaatgggttgcataataaagctaagtgccaggggaagggggcattttgtacttgacatggattggttactgtagtcatattta
ttttcttgccagagtcttctctgtactgcagatcccagcgactggataaaagcatgcagtaattgctctaagtcttcacccaataattagtttttctggt
aatagtaccacttgtccacatcttctgcaacagggctagggggtccacaaggaggcgctgccttcacaatggattccacttcttctgccttggagaggaa
agggctatcttgtaccgtgacgttagtgcaattgatttctcctccttctattcttttagtcaccagtgacttccagtgctcatgggtacttttaattata
ttaatggcaaaatccttatttttaaattctgcgtcaaaggcaaactggttctctggcttcccatctggaactttgtaccgtcggaaccagtcaacggtgg
actccagataattgggtttcagtcgtctgacatcttctatgtcattgtaatgcgcctcgtgccgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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