XB-CLONE-3059559
Clone Name: IMAGE:7847047
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Gene:
MGC147475
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC124012 [+]
CAAAGGATCTGAAGTCTATGCAGGAAGAAGCCGGGCAGAGCCATGTCTGTGATTTACAGCGAGGTTGCTGCGGATGAGAACACCACCGACAGCTTCTGGA
TGCCCAATAACTATGGTAGCACCGTGAAGCGAATGGAAGATGGCTACCGGCTTTGTGATGAAATCGTAGCATGTTTCCAAGACAGAGCCAAGATCGAGCG
TCAGTATGCTCTACAGATGGAAGAATGGACCCGCAAGTGGAAACCTCTGGTAGACAGCAGCCCTATGTATGGTTCTCTCCTGCGCGCCTGGCAGGCCTTT
ATGAGTGCCACAGAACGGCTCAGTGAGCTACATACACAGATCCAGAAGACATTGGTAGCAGAGGATGCAGAGAAGATACGCACGTGGCAGAAGGATACAT
ATCATCGCAAGATATTTGGAGGGTTCAAGGAGTCCTGTGAGATTGAGAATGGGTTCCACAAGGCACAAAAGCCATGGGCCAAGAAGTTAAAGAAGGTGGA
AAAGTCCAAGGCAATTTACCACAAAGCCTGTAAGAAGGAGCACTTGGTCAGTGTCCGTGAGAACAGCAGCAAAATAAACCCCGAACTTACTCAAGAGAAG
CTGAAGAAACTCGCCGAGGATCATGAGAAATGCACACAGGACAAAGAAAAGGTAAAGCAGAGGTATGAGAAGTCCCTACAGGAACTGAATAAGTATAACC
CCAAGTACATGGAAGAAATGGAAACGGTGTTTGATCAAAGTCAGCAACAGGAGCAGAAGAAGATTCTCTTTCTCAAACAGGTCCTGGAGTCTATGCACAA
ACATCTGGATATCACCACTAATGAGAGCGCAAAGAAAGTGTATAGTGATTTAAATGATGCGATTGTCGGGGTAAATGACCAGGAAGATCTAAAGTGGTGG
AAGAACAAGCATGGGCCTGGGATGATCATGAACTGGCCTCAGCTGAAGGAGTGGACTCCGGACACAGAGCAACAAATTGTGAAAAAGAAGAAAGTGAAAG
AGACAGACAAGGTGAAGTTATACAGTGTAACGCCTACAGAAAGCAGCCAAAATAAAGCTCCAATCAATGTTCCTGGAGTACGCGTGCGAGCCGTATACGA
TTATTCAGGGCAGGAATCTGACGAGCTCAGTTTTAAATCAGGTGATGAACTCACAAAGATTGAGGATGAGGATGAGCAAGGCTGGTGCAAAGGAGTGACA
GATAAAGGACAAGTAGGACTTTACCCCGCCAACTATGTGGAAGTGATTTCTGGATGAGGAGGGTTACAGTGGGTTTGAGTAGGAGAAATGCAAAGACTGA
ACGCAGCAGGTTGCTTCCACCAGTGAGTTAAAGATTTCTGCAAGAACAAATGGAGAAAGTAGCAGCAGCCTTACCTTGTAGTTACTCCTCAACAGAAACC
CTACAGGAAATACCACTGCTCTGGCATTGCCAACTCACAGTATTCTTCTCTTTTATATACCAGAACTAAAATATATCTTGACTGACACGGAGGGCATATC
TACTGGGCTGCAGTGTCCCCAAACCTCAGGGGTTTCCCAGCGCGCTCAACGCCAGATTATTGTTTAACAGACCTGTGGGTGCAGCCGAGCACCCATCCAT
GCTTTTTTACATGAGTGCCTTGGCCTCTCTCAATGCACAAGCCAAGGGCTACAATAATATAACCCCGTATGCCTTAGTGCAATAACACAGCAGCCCAGAA
GAAGCTTTTGTTACCTTTGTGTTTGCTGAAGCAGCGAAGGATATGATTAAAGTCTCTGTACCAATCTTAAAAAAAAAAAAAAAAAA
5' EST: DT530855 [+]caaaggatctgaagtctatgcaggaagaagccgggcagagccatgtctgtgatttacagcgaggttgctgcggatgagaacaccaccgacagcttctgga
tgcccaataactatggtagcaccgtgaagcgaatggaagatggctaccggctttgtgatgaaatcgtagcatgtttccaagacagagccaagatcgagcg
tcagtatgctctacagatggaagaatggacccgcaagtggaaacctctggtagacagcagccctatgtatggttctctcctgcgcgcctggcaggccttt
atgagtgccacagaacggctcagtgagctacatacacagatccagaagacattggtagcagaggatgcagagaagatacgcacgtggcagaaggatacat
atcatcgcaagatatttggagggttcaaggagtcctgtgagattgagaatgggttccacaaggcacaaaagccatgggccaagaagttaaagaaggtgga
aaagtccaaggcaatttaccacaaagcctgtaagaaggagcacttggtcagtgtccgtgagaacagcagcaaaataaaccccgaacttactcaagagaag
ctgaagaaactcgccgaggatcatgagaaatgcacacaggacaaagaaaaggtaaagcagaggtatgagaagtccctacaggaactgaataagtataacc
ccaagtacatggaagaaatggaaacggtgtttgatcaaagtcagcaacaggagcagaagaagattctctttctcaaacaggtcctggagtctatgcacaa
acatctggatatcaccactaatgagagcgcaaagaaagtgtatagtgatttaaatgatgcgattgtcggggtaaatgaccaggaagatctaaagtggtgg
aag
3' EST: DT530854 [+]aagattggtacagagactttaatcatatccttcgctgcttcagcaaacacaaaggtaacaaaagcttcttctgggctgctgtgttattgcactaaggcat
acggggttatattattgtagcccttggcttgtgcattgagagaggccaaggcactcatgtaaaaaagcatggatgggtgctcggctgcacccacaggtct
gttaaacaataatctggcgttgagcgcgctgggaaacccctgaggtttggggacactgcagcccagtagatatgccctccgtgtcagtcaagatatattt
tagttctggtatataaaagagaagaatactgtgagttggcaatgccagagcagtggtatttcctgtagggtttctgttgaggagtaactacaaggtaagg
ctgctgctactttctccatttgttcttgcagaaatctttaactcactggtggaagcaacctgctgcgttcagtctttgcatttctcctactcaaacccac
tgtaaccctcctcatccagaaatcacttccacatagttggcggggtaaagtcctacttgtcctttatctgtcactcctttgcaccagccttgctcatcct
catcctcaatctttgtgagttcatcacctgatttaaaactgagctcgtcagattcctgccctgaataatcgtatacggctcgcacgcgtactccaggaac
attgattggagctttattttggctgctttctgtaggcgttacactgtataacttcaccttgtctgtctctttcactttcttctttttcacaatttgttgc
tctgtgtccggagtccactccttcagctgaggccagttcatgatcatcccaggcccatgcttgttcttccaccactttagatctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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