XB-CLONE-3059553
Clone Name: IMAGE:7847143
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Gene:
atp2a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530859 [+]
catcttcggtgaggatgacgatgtgtctagatgtgccttcactggacgtgagtttgatgacctgccccctacagaacaaagagaggcctgcaagcgtgct
tcctgctttgccagagtggagcccacacacaagtccaagatcgtagagttcctgcaatccttcgatgagatcacagctatgactggtgatggtgtgaatg
atgccccagctctgaaaaaggctgagattggtattgctatgggctctggtactgctgtggccaagacagcctctgagatggtgttggccgatgacaactt
ctccacaattgtggctgcagtagaagaaggccgtgccatctacaacaacatgaagcagtttatccgctacctcatctcttccaatgtaggagaggttgtc
tgtatcttcttgactgctgctctgggtcttcctgaagctctgatccctgttcaactgctgtgggtcaacctggtcacagatggtctgccagccacagccc
tgggctttaaccccccagatcttgacattatggacaggccacctcgtagccccaaggagcccctgatcagtggatggcttttcttccgttacttggccat
tggaggttatgtgggtgccgccactgttggagcagctgcctggtggttcatgtatgccgatgatggacctgaagttactttctaccagctgagccatttc
atgcagtgcactgaggaaaatgctgactttgagggtctggagtgtgaaatctttgagtctcctgtacccatgaccatggctctgtctgtgctggtcacca
ttgaaatgtgcaatgccctcaacagtctgtcagagaaccagtctctggtcaggatgcccccatgggtgaacatctggctgttgggctctatctgcttgtc
catgtccctcc
3' EST: DT530858 [+]gagagtttaaattattctttaattttaataaatagctataagagtttcctcgtagatttctcgcgtccgtatctctgctacaagcggcaactccctcgcg
gggctggagagggatcaacaacgccacactgcactttaacaccgaccgtgcgttcttctctctcgccgagatcgactatgatttagctctgagatgtccg
gattggggagaccggttctatatgtacagtatattggtgcggttatgggggggggggggtgaatccctttataaaaagggtcgtgaatggaaaattaagg
cacttggctaacagaacaacagcccgacaattccatcacccttggggggcagatttgggagacgcccaagttggaaggctctggagcggggcgttggggg
gaaagggttggcagtgatgaagtaaaaaaaaatgagttattgggggggcagaaaagtaaaaatgaggatttgggctgagatctgatttttgtaatcagta
caaagttgagttccctttactttactttttttctcttaggcaggaacaaggtggtggagtggtaaagattatgcttccaggtagttacgagcaacaaatt
tcaacagttcatccaacaggatgacagggatggaaatcttcaggacaacaaaccactgagtgaagttcagaggagtcaacttgaagatcattggaagtgg
ctcaacatagaggataaggaagtggagggacatggacaagcagatagagcccaacagccagatgttcacccatgggggcatcctga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: