XB-CLONE-3059450
Clone Name: IMAGE:7847009
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Gene:
cyc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530927 [+]
ctgcaggggggacgggcggcctggccggtggctcctcaggccaacatgtccttctccactctgtcccggggcaggaaggtggctctctccacattaggta
ttttggtcgccggaggctccggtttggcgtttgcactccaccaatcagtgaaagccagtgaattagagctccacccaccctcctacccatggagccacaa
cggactcctctcctcgttggatcacgccagcatccggcgcggctaccaggtgtacaaacaggtgtgcgccgcctgtcacagtatggaatacttggccttt
aggaacctcatcggcgtctcccacacggaagcagaggccaaagcgctggcggaagagtttgagattctggacggcccggatgagaatggggagatgttca
cccggcccgggaagctgtcggattatttccccaagccgtaccccaatgacgaggcggccagagcgtccaacaatggggcgctgccccccgacctcagcta
cattgtcaatgccaggcacggcggggaggattacgttttctctctcctgactggttactgcgacccccccgctggcgtcagtttaagagaaggactgtat
tacaacccgtacttccccggccaggccgtcgggatggcgccccctatttataacgaggtcctggaatttgatgatgggaccccggccaccatgtcgcagg
ttgctaaggacgtctgcacattcctgcgttgggcttcagaaccggaacacgaccagcgcaagcgaatgggccttaaagtgctgatgatctcttccatcct
gatcccactcatctactacatgaagagacaccgatggagcgtgctgaagtccaggaagattgcctaccggcccccc
3' EST: DT530926 [+]ggtttaaagatatatttttgtttaatttaacgagcccataatgggatgtaatggacacaatcgtttcttcgcacgagtccctttttcctcctcggtttgg
ttctttccggacaaaaacagtgaaaacgcagagaagcaggaacaaaaaattcccccgagacaaaaaataaacataaaaaaaacaaaaagatacggacgca
gcgcctgtgagttacggagccgtgtccggatctacttcctgctcggaggagcaaagggaccgttgcctggcaaatcatccatggcatctgctgaggccaa
cgcgcagggggaggggcttcccggtgctgagctgtcggctgccgttacttggggggccggtaggcaatcttcctggacttcagcacgctccatcggtgtc
tcttcatgtagtagatgagtgggatcaggatggaagagatcatcagcactttaaggcccattcgcttgcgctggtcgtgttccggttctgaagcccaacg
caggaatgtgcagacgtccttagcaacctgcgacatggtggccggggtcccatcatcaaattccaggacctcgttataaatagggggcgccatcccgacg
gcctggccggggaagtacgggttgtaatacagtccttctcttaaactgacgccagcgggggggtcgcagtaaccagtcaggagagagaaaacgtaatcct
ccccgccgtgcctggcattgacaatgtagctgaggtcggggggcagcgccccattgttggacgctctggccgcctcgtcattggggtacggcttggggaa
ataatccgacagcttnccgggccgggtgaacatctccccattctcatccgggccgtcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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