XB-CLONE-3059200
Clone Name: IMAGE:7847176
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Gene:
hnrnpd
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531092 [+]
ctgaagacgatgaagccatgatggaggctactactgctgagacagaggagactgggcaaaacgaaggggtgaagattgatgctagtaagaccgaagagga
tgaggggaagatgtttataggaggccttagctgggatactacaaagaaagacctgaaagattatttttcaaaatttggagaggttgtagactgtaccttg
aaattggaccctatcactggaagatctcgtggctttggctttgtattgtttaaggaatctgaaggcgttgacaaggtaatggaacagaaggagcacaagc
tgaatggtaaagttatagacccaaagagggcaaaggcaatgaagacaaaagagcctgtcaagaagatatttgtagggggattatctccagacacacctga
agagaagataagagagtattttggaacgttcggagagattgaagccatagaacttccaatggataacaagactaacaaaagacgtggattctgcttcatc
acatttaaggaggaagacccagtaaagaagataatggaaaagaaataccacaatgttggcctcagtaaatgtgaaattaaggtggcattgtcaaaggaac
agtatcagcagcagcagcagtgggggacaaggggtggaggctcatcatctaggccccgtggaagaggaggtgtaagtcagacctggagccagggatatag
taactactggaatccaagctacagcagttatggatacaacaaccaggggtatggaggttatgggaactatgactactctggttacaactactatggctat
ggagactatagtagtgagtatccataaatttgacaaggcttgtgatggaagcattaaaatattaataggtcattgtttttcat
3' EST: DT531091 [+]tttttttcatttttagccctttatttaacagcaaattttgtacatgaaaaacaatgacctattaatattttaatgcttccatcacaagccttgtcaaatt
tatggatactcactactatagtctccatagccatagtagttgtaaccagagtagtcatagttcccataacctccatacccctggttgttgtatccataac
tgctgtagcttggattccagtagttactatatccctggctccaggtctgacttacacctcctcttccacggggcctagatgatgagcctccaccccttgt
cccccactgctgctgctgctgatactgttcctttgacaatgccaccttaatttcacatttactgaggccaacattgtggtatttcttttccattatcttc
tttactgggtcttcctccttaaatgtgatgaagcagaatccacgtcttttgttagtcttgttatccattggaagttctatggcttcaatctctccgaacg
ttccaaaatactctcttatcttctcttcaggtgtgtctggagataatccccctacaaatatcttcttgacaggctcttttgtcttcattgcctttgccct
ctttgggtctataactttaccattcagcttgtgctccttctgttccattaccttgtcaacgccttcagattccttaaacaatacaaagccaaagccacga
gatcttccagtgatagggtccaatttcaaggtacagtctacaacctctccaaattttgaaaaataatctttcaggtctttctttgtagtatcccagctaa
ggcctcctataaacatcttcccctcatcctcttcggtcttactagcatcaatcttcaccccttcgtttttgccagtctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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