XB-CLONE-3059170
Clone Name: IMAGE:7846988
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Gene:
atp5me
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531112 [+]
ataaagttcaccagatattctgcattgctggttgggatgatttatggcatgaaaagatatgactatttaaaaccaattgctgaagaagaaaggaaggtag
aagctgaagagaaaagacagcgagaggaagctgagcgaattgctaaggaaatagcagctgctaatgaagataccattctgaaatgaatttccatccatta
aaggacagtgtaccaccatattgtgtttctttgtttattgctcctgggttgcttatgtaaaagaaatgtaccataatataaacagccaagaaaaattatt
gcttattcttgtgttatgtatgttttctttttgaaatatgaggtttttttgttatcatacatacttaagtcttctaaatcttagaatgatacagtaatgt
atttctcttgaaatgtgaatgtcgctgaataggttaacccactagttttcagcattttgggcaaactccctagaaggaattcagcaccgtcgataagaaa
cctaggggctgatttactaatccacgaattcgaatccgaaaaattcgggttggaaactaacattttgtgatttttttgcaatttttttcgtcgccgttac
gactttatcgtaaattgttgtgactttttcatagccgttacgacttgcgcgaaaagtcgtgactttttcgtaggagttacgatttgctcgtatattgtcg
caactttttcgtattgagcgctcgtaaatggcgggcaaaactttcagactttgcatgattttggaagcctcccataggactcaatggcactctgcagctc
caacctggctcnagaaagtcacgatactgaagcttgaaaaaatccgaaacttttgtactcggcgtga
3' EST: DT531111 [+]acaaatttgccaccgctttttattagaactgcaaaaatgcattgctagccaagatagctaattagcattcaattctgttgtcccacttttcttgacttta
gactaaggggctgatttactaatccacgcaaattgtcgcgactttttcgtagcgttacgacttgcgcaaattgtcgcaactttttcgtagccttcacgcc
gagtacaaaagtttcggattttttcaagcttcagtatcgtgacttttcttgagccaggttggagctgcagagtgccattgagtcctatgggaggcttcca
aaatcatgcaaagtctgaaagttttgcccgccatttacgagcgctcaatacgaaaaagttgcgacaatatacgagcaaatcgtaactcctacgaaaaagt
cacgacttttcgcgcaagtcgtaacggctatgaaaaagtcacaacaatttacgataaagtcgtaacggcgacgaaaaaaattgcaaaaaaatcacaaaat
gttagtttccaacccgaatttttcggattcgaattcgtggattagtaaatcagcccctaggtttcttatcgacggtgctgaattccttctagggagtttg
cccaaaatgctgaaaactagtgggttaacctattcagcgacattcacatttcaagagaaatacattactgtatcattctaagatttagaagacttaagta
tgtatgataacaaaaaaacctcatatttcaaaaagaaaacatacataacacaagaataagcaataatttttcttggctgtttatattatggtacatttct
tttacataagcaaccccaggagcattaacaaagaaacacaatatggtggtaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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