XB-CLONE-3059137
Clone Name: IMAGE:7847134
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531134 [+]
caaacatctaacaatccacaatgggaaaggaaaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaactggacatct
tatctacaaatgtggtggtatcgacaagagaaccatcgaaaagttcgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttg
gacaaactgaaggccgagcgtgaacgtggtatcaccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccag
gacacagagacttcatcaagaacatgatcactggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtat
ctcaaagaacggacaaactcgtgagcatgccctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaaccc
ccatacagccagaaaagatatgaggaaatcgttaaggaagtcagcacatacatcaagaagattggttacaaccctgatactgttgcctttgtacctattt
ctggatggaacggtgacaacatgcttgagcccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactac
cctgctggaagctcttgactgcattttgccaccaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggt
actgtaccagttggtcgtgtggagactggtgtcc
3' EST: DT531133 [+]tttttttcttttactttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaacactaataact
taaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactct
gatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgcttt
ctgagcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaa
cgaccaagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggat
tgtcttccagtttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcata
tccagcaccaatctggcctgggtggttcaggatgataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttg
ccacgacggacgtccttcacagaaacgtttttcacgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcattttcacagatt
taacttcagttgttacattaacaggggcaaaagtaac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: