XB-CLONE-3058960
Clone Name: IMAGE:7847198
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Gene:
trit1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531251 [+]
ctcattgccgcttgtagtggttctcggggctacggggaccggaaagtctaaattggctgtgcagcttgggcggacacttggtggggaaataatcagcgca
gactccatgcaggtctacaagggactggacattatcactaataaggtcagtgcagaggagcagaaactgtgtacacaccacatgatcagttttgtggatc
ctttggtcgccagttacacagttgtagactttcgaaacaaagccgtgactctgattgaagatattttctcaagaaacaaaattccaattattgtgggagg
aacaaattactatattgagtctttgctttggaaaattttattgaatacggtaacaaaagatggaataaaaagttccataaacagcaatgaaaatacacag
acagaaggtaataggaaagaagaacttgagaaattagacagccacgaactccacttgcgtctcaagcatgtggacccagagatggcatcaaagcttcatc
caaatgacaaacgtaaaattgccaggagcctgcaggtgtatgaggaatctggcatcccccacactgaacatttaaggcgtcagcaggaagaaaatggagg
gggacctcttggtggagccttgaggtaccagaatccctgcattttatggcttcatgctaatcaggaagatttaaattctcgcctaaatgtcagagtagat
gagatgctgaaacttgggttgatcgaggagctacaggactttcacaagcattacaatgagaagctaataactcaatccaggcaggattaccagcatggca
tctttcaatcaattggcttcaaggagtttcatgaatacctaataagtgagaaaagtgattcaagtgcaaaggaggtcctgcttca
3' EST: DT531250 [+]tttttttcacactctttgaaacctgggtgcacaatcgctttttcacttgggtgtttccaagatcacactgaccttctaatttcctcttcttttttgcatt
attaagatgagatttagacttgatgtgagctgcccattcccgatcaccaattatgactctactgcacagatcacatatgcgactacttcgcttattcaca
gtttcatcacagtcaagaagcagaggctctatcgctggatcttctccctgtagaaaactgttgacaatctgcagagcaggtgacagaacacactggtccc
aagcagagacatcagtcacgtctaagcggtacacacggggcacattaggtcctgggcgcttaaggaatctgttctgaacccattgattttgtttctttgc
atatttctgtgttctttgcttcagactctcaatacctaaaaacaagaaaaaaaaactgttagtacagtcactccatatattacttaaacaatctcagcaa
aatattttacctttctgaagcaggacctcctttgcacttgaatcacttttctcacttattaggtattcatgaaactccttgaagccaattgattgaaaga
tgccatgctggtaatcctgcctggattgagttattagcttctcattgtaatgcttgtgaaagtcctgtagctcctcgatcaacccaagtttcagcatctc
atctactctgacatttaggcgagaatttaaatcttcctgattagcatgaagccataaaatgcagggattctggtacctcaaggctccaccaagaggtccc
cctccattttcttcctgctgacgccttanatgttcagtgtgggggatgccagattcctcatacacctgcaggctcctggcaattttacgtttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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