XB-CLONE-3058916
Clone Name: IMAGE:7847202
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Gene:
XB1001290
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531280
[+]
atacattcaggagattaagcagagggaccaactcatacagcagatcaactcaaaggcaatggagtcagtgcagatgaacgcgatgctgtcggcccagctg
aacactgtctcccagggactgaaggaatctcagttgagatacagtgacctgcagaaccagtactacacacttcagagggacctgcaggctactaaccaga
gtgacatccgcactgaagtcccccctggtgctccgcaggagagggcaagcgtcctggtggagatagaaaattctgagctgattgacctaagaagaaggct
tgtggagagtgagcatctgtacgactccattcagcaagagctgtcacagctgtcggagagattcgcagaggaaaggtcgcggagagaggcagcagaagat
gctctacatctatcagagcaacataataaaagcctggaaactaggcctttatccagagaatacgagttctcccttcagatggagtcggaagatgaacgag
aagcgctgataattgatccgacacagcatgtcatggttagaaagatgaaaggaggcgcactttctctgcggaggtggttgcgtggccgaagcctgtactg
ttccaagttactgacttccagatcgaaagcccgttacctgttcctcacctacctgttggtgttacacgccgccgtccttatgtgcctgactggcgtgtta
tagccgaactctcaggcaccagttttaggcttgtgcggctactcaagggttaacactgaacaggtattatgtcccgattatgggacgggctctgcagcac
aacgtattttgctggattttgcactgcatggacgaataagagcggtacaggggtcgcgggtttgctgtttatc
3' EST: DT531279
[+]ggtttaagatttattttcagatgagaaaccaacagatcaataagggaatttattttattgcactttaagcttccgtgccacacaccaagaagcagagaca
cacccaatgcacagggcatgggacttccattgggtccaagtccttaaagggatactgtaatcaaaataacggtttcagtgcttgggtttcaggggaaggg
aggattttgaaagaggtagagtttattaaccgtgacttggggtaacagtgttttcttaaggaaatggtgactgtcggatactttccagtttgatgttgtt
actatgcatttgactgcagggcaaccaagtttactgatatgagcattattacacaggattgggaagtatttgctatactttcctctggggtgactcctgg
cattttgtctcccccgggtgccttatgaagcacatggggtaactgactgatacggcagtataaagcccatgattcataaggccaatctgcaaagccgctt
tccgttcagcagtgcagactgggacttagattgctgttgtgatgacagtatccctttaatacacggagcagggtcctcaaacacatggccagttctttat
aactatcccaaggcattgtgggagttgtagttctctgcacctggaagggtgggaaagccaccaccgttaggcttacaaaataatatctgcatagagaaga
aaatcaattttcccaccatttcaagtagaaatcatccatacacacctgaagagcagcaatttctatacagtaggaactagaatctctttagcaaatataa
tagaatctattatggaacaagcgagatatacagaagtcttaattac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: