XB-CLONE-3058904
Clone Name: IMAGE:7847012
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Gene:
ank2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531288 [+]
catctgaatttagcaaggaaagtaaccaatctaaaagtgcatgtatcacatcaaggtgctctaaatgacttcacagtctaaaccatctaaacaaatgtaa
tatttgttaaaatattaagaggaatctaattagatagccaatatgctagctgcttatataaaggcatatatacacacatttaaatattctagacttgaca
aatggcaagcactactgtacaactaccaagctctttaataacaggcagttgatgccgttcagcaattgcaccccacagtttggtaaatggataatgtacc
aaggtggcccttttgaatggaaaagcatccaaggtgcagtcgacaaatgctaaacaacttctagttgaagatctagtcaaaaggtagtcaaagctagtct
agaatctaggcagcagatcacagttgagaggtctaggttctagtcatcgggtatctagactgatgtaactaaagcagttacttgctggcagcgatgcttt
ggcaaaccacattgtcttactactagtgggaacgtcaggtggcaaaaggactgtaaatattgtaatggataagacatttccataaacgaaaaactatata
actcactgtgttttatcaggatttcctctggcacatgcatgtggcattaaaaacaagaaataaattcctataataatgtgtggagcttgttaaccctttt
tagcactaagcaagcaggtttcacaattaagtcctttcctatttgtgcatttcatttttccctcccaacccaaaagctaagaactcaatgcaggttttgg
tcttgctctttttttatgtttttatgtctctgaaccaat
3' EST: DT531287 [+]ttttttttacatttaacactttatttaattaaatcagccattgtacacattgcagctatgtattgttagtgtaaatttttgccgttttttaactaagaca
tgccctcgtagatatattaaattagtgttatcaccatgggaacaagatgctgaattgtcaactgaaatgtcactctcacagtagttgtttgataacacgg
caaaaaataaaatcttaacccataaagtgattaaccatttatgctgttacacagacatctgcactgcaaatagcacaacagaatgtttccctctagactt
agcccagcattgaccaaggctattgtgagccatgagagagccgtgatgtgaactgtcctttggccccatcactagcatacaggaatatccacgtaactta
tcagagctctttccggcacaaagaggggccgccactctgttggagaaggtgtatgtttaactcatctttgctttctgaaaaagtaatttagcgatatttc
tgaaagaataaagtgatgctacacagtaggtctatttttaaacaaatccaaggatttttaagaagtgtgtacttttcacggtacttttccatttacagaa
ctgacattcatcggactgtgtgggtcattttgcttacttcctaacaaaaggcctaatttctttgttttatttataaattagcgacatctagttggttatg
tgaatattttgttttttttcgataaccaatctatacttacattaaataagtgggttcaaaatgtataatgcttatacatatttatatatatatatatata
tttatttgtatgttaaaaggctacagagttcaaatcagaaagtaaataaagtacag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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