XB-CLONE-3058881
Clone Name: IMAGE:7847014
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Gene:
pold2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531303 [+]
cgatccggggggagagttaaagtaatgtttacagatctagcaatttctcagggccccggccttcttacagccccttctgaggtccaggccacgtttacac
gagtgtctaacacacagtatgctaactgctcgagtgtattccgattgggcgaacgcacctttacccgtcagtatgcgcacatttatgccactcgcctgga
acaaatgcgcccgcttttaataaagagagcaaagcagctatggggtgatgatgtagctgtgcgaaagctgtgtgagctgcagggggaagagaagtgttgc
gtggtgggaaccctgtttaaatccatggaactccaaccctcaatcctgcgggagataagtgaagaacataaccttctgccccagcctgcaaggcacaaat
atatcagtgattctgacgagttaatattggaggatgagctgcagagaatcaagctggagggggctacagatgtgcaacagctcgtgactggtgcagtcct
ggcagttcttggttcagagggggatgctggaaagttcatggtggaagatttttgtctgacttctcttcctgcacaatctccactccccaggctaagtgag
gacaggtttgtgctgctgacctctggccttggcttaggtgggggcagcggggacagcctgatgggactgcagttgttactggacctagtcactggccagg
ctggtgcagaggaagatcaaggttgtgcggctcggatatcccgtgttatcttggctgggaacctactgagtcagaacacacagggcaaggattctctcaa
taggccaagtatctcagcaagaaaactcaggct
3' EST: DT531302 [+]gaaagagagaatgttttattccctcagagtcatttcattaaaaaaatatatctataaggaaacatcataagggtgtgtattccacattactttcttagta
aaaaaaaaatggatatgtagagtgtaacacagtgaaccttagtgctcagaaacttacaagtgctccacttattggcattccatagcttaaacatagacag
cagatcaatcacacaattcctcatctgcactgaaaccagagaagctgactggctggcactgaagtgtacgcaaattcaccagacaggcagtctgcgtgct
gcagaattctgggactgtgagcagcagaacgcgctgaccttctttacctgtgacctctttgcaactgaacttgggggcacttccgcaaaaatacacgtga
gggcagttttgcaagatgaacggatcagacttgtagaagggatagcagccaagtgtgtcaggggcagtggggcacaagtgtccaacctgcagtgtccact
ctaatatatccagataatcctgcatgctgctgtacttatagatgtcactgatattctgaccagatgtccccaggaacctgacaccatcaatctctgcctc
atacgggtttgtcacaagctgaagagtggagtatacagcagactgtgggaacatacaccggtgcaagggctgctggggcaggatgtagtttgtagggtca
aacgcaccaggcatcacatccacagacactgagccgctcagctgaaggaggatctcatccaacatcttcacagcttccacactggcagcctgag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: