XB-CLONE-3058872
Clone Name: IMAGE:7847158
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Gene:
mrps17
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121535 [+]
CCGCTCCTGTCCTTCCGTTTGACCTTTTTGTTTTATTTATCCCGTATACTTTAATCCATGTGTTATACATGTTAATACAGTTAGAAAGAATTGTGCTGCA
GCTGTAAGGACGTTTGGAGGCTGATCTGTTACATTTAGTTGTGTGTGTCCCCGCTGGAATTCACAGGTTCCTTTGTTGTGATGTCAGCCATACGAGCTGC
AGTCCACGCCAAATGGATTGTAGGGAAAGTCATTGGAACAAAGATGAGAAAGACCGCAAAAGTCAGAGTGACCAGGCTGGTGCTAGATCCTTACTTATTA
AAGTACTACAACAAAAGAAAGACCTACTTTGCACATGATGCCCAACAGCAGTGCACCATCGGAGACATAGTTTTGTTAAAAGCGCTGCCAGAGAGGAGAT
CAAAACATGTGAAACATGAACTGTCTGAGATCATATTCAAAGTAGGGCGAGTGGAAGATCCCCTAACTGGGAGAAGGTGTGCTGGGAGCATGTACCTCGA
TAGTCTGACGACTACTGACGATTCAAATGTTGCAGACCTTAATGAACAGCTTCAGAGTCTGGATATTAAAGACACTGCAAGTCAAGACAAAGCAGTGGAT
TCCTAATGTTTTGGAATCATGTTGACTGTGTTAGTAAAAAAAAAAAAAAAAAA
5' EST: DT531309 [+]ccgctcctgtccttccgtttgacctttttgttttatttatcccgtatactttaatccatgtgttatacatgttaatacagttagaaagaattgtgctgca
gctgtaaggacgtttggaggctgatctgttacatttagttgtgtgtgtccccgctggaattcacaggttcctttgttgtgatgtcagccatacgagctgc
agtccacgccaaatggattgtagggaaagtcattggaacaaagatgagaaagaccgcaaaagtcagagtgaccaggctggtgctagatccttacttatta
aagtactacaacaaaagaaagacctactttgcacatgatgcccaacagcagtgcaccatcggagacatagttttgttaaaagcgctgccagagaggagat
caaaacatgtgaaacatgaactgtctgagatcatattcaaagtagggcgagtggaagatcccctaactgggagaaggtgtgctgggagcatgtacctcga
tagtctgacgactactgacgattcaaatgttgcagaccttaatgaacagcttcagagtctggatattaaagacactgcaagtcaagacaaagcagtggat
tcctaatgttttggaatcatgttgactgtgttagtaaaaaaaaaaaaaaaaaa
3' EST: DT531308 [+]actaacacagtcaacatgattccaaaacattaggaatccactgctttgtcttgacttgcagtgtctttaatatccagactctgaagctgttcattaaggt
ctgcaacatttgaatcgtcagtagtcgtcagactatcgaggtacatgctcccagcacaccttctcccagttaggggatcttccactcgccctactttgaa
tatgatctcagacagttcatgtttcacatgttttgatctcctctctggcagcgcttttaacaaaactatgtctccgatggtgcactgctgttgggcatca
tgtgcaaagtaggtctttcttttgttgtagtactttaataagtaaggatctagcaccagcctggtcactctgacttttgcggtctttctcatctttgttc
caatgactttccctacaatccatttggcgtggactgcagctcgtatggctgacatcacaacaaaggaacctgtgaattccagcggggacacacacaacta
aatgtaacagatcagcctccaaacgtccttacagctgcagcacaattctttctaactgtattaacatgtataacacatggattaaagtatacgggataaa
taaaacaaaaaggtcaaacggaaggacaggagcgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: