XB-CLONE-3058764
Clone Name: IMAGE:7847503
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Gene:
capn11
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531380 [+]
ccgagggacacattgtgacagagcagcaccattgtaccgagcggagccggtgggaccgacagactgaggggattcgccgggcacagaacagtggaatgat
gccgttcgggggtatagcatcgaagctgaagatcgagcggctgaaggcgtcgggcgtgggctcccacgattgtgccgttcggtaccagaaccaggactac
gagagcctgaagcagcagtgccgggagagcggcagcctcttcgaagatccaaacttcccgcccattggctcctccctgggcttcaaggaactgggccccg
gaaccagcaaaactaagggggtgcaatggaaacggccccatgatattgctgatgatccgcagtttattgttggtggagccaccaggacagacatatgcca
gggggcactgggggactgctggctccttgctgcaattggctccctgacgctcaatgaggatttgctgcacagggtggttccgcatgggcagggcttccag
gatgactacgccgggatcttccacttccagatctggcagtttggcgagtgggtggatgtggtgatagacgaccgtttgccggtgaaggacggggagttgg
tgtttgttcactcggccgagtgctcggagttctggagcgccctgctggagaaggcctacgccaagttgaacggttcctacgaggccctgtcggggggcag
caccaccgagggattcgaggactttaccgggggagtcgcggaaatgtacgaattgcgcanagccccccggaacctggataagatcattaaacgggcgctg
gacaggnggtccctattggggtgctccatagacataacgagtgcctttgacatggaagccgttacatttaagaagcttgtgaagggc
3' EST: DT531379 [+]agggcgagaggtttcattctttattcctgcagtctggcacaattccgcctctagggggcgacagtacatttgcactcagggaaacctcaaacataactgc
cccactctgaaagcttgtggttctggcccggggacccctgtaagagtcagatgcccccccagtacaagaaggggccccggtatctgtatatacaataaca
aaacgctactgggcggagccctggggccggagtacagtccttacagttgtgcctggcagctgcccgcttggcattggatacattatacgggcagcattct
gattggctggttctgattgcaaaatctttcccccaacaaaggcctctgggaaatgtgtaacctggatgtaacgttggtagcgagtgagcgagggcaccgg
gggcaatgagtgtcagcgagggggcaagagaattatccacacatggtcatcgtcagccactcccctaaactcatctcggctgtgccctccccttggtcca
acgccttgaagaaccggaacatggcctccagcttcacgaggcagcagacgaagttatcgaagtcgattcccatgtcggaatccgcgtagcgagcgaccag
aacctgaatgagtttgttattcagtttgtaccctgtaccctccagggcgatgcgcagctcgtaggcgctgatggtgcccgacttgtccaggtcgtgttgt
cggaaagctgtcagccattggcgaattttgttccacaggatctgaaactcgaccacgcccagtttgccgctgccgtctttgtccatcagattgaccatct
gcctgcaggcctccatcccgaaaccgtccgtcttcagatctttgtgtttggccaccactctgttcaggatcgtcttcagctcaaacacactgatctccat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: