XB-CLONE-3058749
Clone Name: IMAGE:7847361
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Gene:
serinc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531390 [+]
tgatgttcaacaaaaatatttcgtagtgctaaagttgcccttgtacattttacagctattttccttcatatttaggcagcnggcagcacntctagctaca
cnctttccccacacatgaccactattaataaagacaggacaaagactggtcccagtctacgcttgggtgttagtagccaagaatactgcaatacactgca
ttacaaaacaaattcccctcaaggtttatcatccatcaatactgagtgtagcaggacaggaacgtaaaaatatataaacacaaatgtaggaaaacaaaag
caatgacattaatttgacttaaagaaaagcagtgcatctgaacaaggggcatataaaatatccaacagtgatttcttactagtatgaaatagacttaaat
actctagatcagctcagctgtggcaaataaaaaggcagcaatgtatatatattatcaagctttaaagacaggaagtcaaaatctgtttaaagtaagaaaa
cagccaacagttttacattggctcgattaaaggttttcgattatagtcaaatagtatttgagcagaatttgatgttcttggaatgaggtggttagctgaa
gtcacgattggagagaacaattggagctatcagcgtccagaagtagagaagcaggcatacccagctggagctgatcttaaaccacacggcgggccatgta
cttgtcatggttttggtgtctgcagttgggctgtaccaattagtcagagtcatcataatgtacagagaggagaggcagagcatgaaaaggaagagggaat
agttgtactggacgccatccttctcattgtctataacgcgtttcacttctccatcttctgcatcgctgggatctgtt
3' EST: DT531389 [+]ctccggtggagccgagtctgagccatgggtggggtgttgggattgtgctctgtcgccagttggataccatgcctatgcagcagtgcgacgtgcctgctgt
gccgttgctgcccgaataccggcaactcgacagtgacccgtattgtctatgcgtttctgatgctgcttggggccatcctttcttgcataatgctctcacc
aggcatatcggaccagttaaagaaggttcctggattttgtgaagatggctttgggacacaattaccacacgttgatggatacgtgaactgtaacgtgctg
gttggatacaaggccgtctatcgtgtcagtttcgctatgaccctgtttttcctcgccatgagcatatttatgttaggagtcaagacaagcaaagatccac
gtgctgcgatacacaatgggttttggttctttaaagtattggctctggctggaattatggttggtgctttctatatcccagaaggacattttacatccgc
ctggttctggattggcgtttgtggggcctgttgtttcatcatcttccagttaatcctgctcgtggattttgcccattcacttaacgagagctgggtgaat
cgcatggaggacggaaactccaagtgttggtatgcagtgttactatcctttaccattttatgttattctttatctataatcggcgttgtcctcctctacg
tatactacacgaagtccgacggttgcaccgagaacaaattcttcatcagtttcaacatgatcctgtgtgtgatcgtatctgtcatttccatccttccaaa
agtgcaggagcatcagccacggtctggtcttctccagtcctcggtgattactttatatacaatgtatctgacttggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: