XB-CLONE-3058681
Clone Name: IMAGE:7847367
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Gene:
myoz1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531435 [+]
ccgaggcttgttgctgccctctgagagacaagacacaagggagccaaattaataaaaaggaaagaatccgcaagaggacgtttacaattataacatcaaa
gaagctcccatctctcctcctttagctgatctccagttcctgcgaaaaagaattaagaatttgttgtaatgccactgtcaggaacaccagctccaaacaa
gaggaaaaagtcaaccaaaatcatcatggagataaatccatcaagagaaggagagatagaaatctccaaactcaatttagggaaaaagatcagtgttccc
agagatatcatgctggaggaactatccttgctcagtaacaaaggatcgaagatgtttaagcttcgtcaaaagagggtagaaaaatttatctatgaaaaca
accctgacgtattttccgacaattccttggaccacttccagaaatttataccaactgtcggtggccaattaggggctaaaggacagtcaggtgttggagt
aggtggctatggattgctatatggcagtggtcagatgctatccagtatttcagggggacggcatggatctggtggtcagcaacctcctgtacctccacca
aaacctggcagtaagagcgctcctggaggccagtctggaactggaggaagtggtggaaaggattctggtgttgttgatggtagtcaaggaaaagagggac
agtcaggtcaacaggatggaggaagtgggaagcagatcactgtctttaagacatacctctcaccctgggacaaagctatgggcattgaccctaagcaacc
aacacattttactgtcaatttactagagtatggcaaaaaggcagagctttgcaaatataagtcattttacaggagtgctat
3' EST: DT531434 [+]ttttttttaatgttgtaaatgtttattcagtttaacattgcacaaatgaaaaatatcaaccatacagcatgactgaggagccaacagtaaacttatttgg
agactgtccttttatcgctgccacagtgcacaaaacattaagcttttttccttccactatcctaccttgtctattccatttacctctgcccagttccaac
tctgactaacttccagctgaaaaaataccaccataacaaaaaacatttcataaacatacataaaggagctttgctgcattttgaacaaggcacattagag
tgtggcatagttgggtctaatgtgatctgccccattttttaaaaaaggcatataaagatttggcttaggtggtttagatatgacctgtgccattggctgc
tatggcatcacagcatactgcaatgcattgcttgtaccacagatatttcagggatcaaccgccaatgtgtttgcaatatgttatgatgcagaggccagtg
gaactttttacatatgtcagttttcatacaccaacagactgctaacatggttccttggatgtatgatgagtttagagttcttctgtttctccatccactg
gcacagcaatatcaatgctgtagtttgtgttatcaccagatccaagccatccaattggggtcctgttgaaagaaggtctgttggtgatatcttgattgat
gacaaccacaggctcaggctctggagggggggcatcaaaatctggcatctggaaagtcataagcttcgatgctttctcatatccaccaaaaggcatagca
ctcctgttaaatgacttatatttgcaaagctctgcctttttgccatactctagtaaattgacagtaaaatgtgttgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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