XB-CLONE-3058473
Clone Name: IMAGE:7847481
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Gene:
otud1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531572 [+]
ctctaacatgcccgccttctcctgcctccccagcgctgcgctcggcgctcacacccacttagagtccatgtgcaccccggagaggatagtgcccgtccgc
ctcctgcagcgaagccctaacgggcagcagcgctacatctccgaagccacaatgcacctgtctgcgccccaggagcaagccctagcccccgcccagcagc
cccaggatatccatgcatggctcagcgacatggtgcaggagacgatccccaggcccgacaccatcgcggctcccatagaagatcaatgctacgaaagtaa
cagcagcagcagcggcggcttttggcacccggaaagcccggagacgacggccgcacaatcgcaggccggtaacagggagcggttggccttacacctggcc
gagctggaaaagcaggacaagtacctgcgggaggagcagcgctatcgcttccatatcatcccggacggaaactgcctgtaccgggccgtgtgcaaggcgc
tatacggggagcaaagcctacacgccgagctgcgggagcgcaccgtgcactacgtggccgatcatctagacattttcaacctcatcatcgagggcgacat
tggagaattcctcatcaacgccgcccaagatggggcctgggcgggatacccggagctcctggccatgagtcacatgctggaagtgaatatccgcctgacc
accgggggcaggcccgagtgccccaccgtctccaccatggttcatcggatcggaggcgaggatcccagccggcctagtatctggctaagctggctcagca
acggccactacgacgcagtattcgagcagcccctgcccaac
3' EST: DT531571 [+]aattgttttttttttttttttattaactcagtactaactgggaatgtaactaatcagcagaggatagaaatcgccattcttacaaaatcattaagtataa
acaacagtaataaatactgaaccgcaatggggagaaaaaaacaaatgtaaacaaacattgtcatgtggcccataagtttcctacacacttggatctcacc
agccaaaaagctaaagtccagtcatttccactataacaaaaacaaatgaacacaaaatgttaacccttcatatagaaacaggtttgtaaataacaactga
aaatgtcttagatataaataccacatgtccatttattattaactggcaagcggaagtctttgtccattaaaatgatgttacaaaaaaatagttaaggcag
aggcatccaacctggggccctgcaactttagctaaactacaactcccagaagccttgacagatgctggaaatcgttattggggggggggtgaaggtagga
catccctgagttaaggcatcaagtggtaaatgagaaggtttaaagtgcagcaaacttccactggtgagagtccaggtatatacacaacacagtgggcctg
gcaactggcagtcagctggaacaaggcttggcacaccctgtgttgcatggactgcagaacgtattaggaaacactgactggtacccaaagagtcagagag
gccattggtagtgcctgtgatatttcctgctccagacactggcaatttgaggcctttggtgtaagggtgccaagtgtgtccctttagaactgtgagccta
acgcaaagccccagctgcttgtaacgggccccggaatgcaaggccc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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