XB-CLONE-3058446
Clone Name: IMAGE:7847531
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Gene:
ehbp1l1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531590 [+]
atcagctgagtcttccaagaaaaatgaagcgccattgctggaataaagcttcttcatcgtatttaagctgcactgcgcagaatccaactaaattaacttc
acttgaagctgaatgcaaattacacagataaaatgatcttcccatcgcaccagtactgacgatgcaaaaaaaaatttaaactgcttcagctactttcaaa
tagctgtgaacttgatgagatctttgacagaaggatggtgagaagcaaaatcctcttctaaatattactcaggctttatgttcttccacttgctgctaag
gtcatagtaatgatctatattaattattcaaatgtgtttttttgcatgaataggttatctttgcaagtattataagcaattatcttatttcatagaattg
tttttgtaatttgtgatttttttgattaaggtaatatccttttaaatagtgacagattccagctaaagtctatgcattgcattcataggtatattccaga
tctgctcaaattatcttcaatgataataaatgtcatggacaactcatggatctggaaaataattaattgcttctttgtatcaataaacagtactatactg
tatgtccttgtaaaaattttgagcttattgctgcctattggaaaccagattggcatcaacaacatttactagaaattgtataatgaacctttctacatgt
cactgcccctgtttagggtgttcgccattagcaaggcaattaaaagcactgcatataggtgttggtggtatataaataaataaaagtaataatttataat
gggcctaatctggccccatatttttataaaaagaatacaaatttgtatatccttcaggcataactcttttgagta
3' EST: DT531589 [+]catgatcaatggcacattttatttaacacttcaagcaaagcaacaatatcacaataaattcccagaatttgtttcgtaccgtgacagtaaaagtgaaaca
caatgctacaaaaaaaggcttctgcgtgcattccattctagctagtacacataaatgagtgcaagccacatccagtactgtggctacaggcagcaaaact
gtggaaatagaaggaccaaagctctttgctaatcacaggcatattctagagactcatcataggcacatcaggtttatagaacataagctattcaggcaaa
gcattactctttaaaaagttgaattcctcaaaatgtatatttgcatgtcatagatggaatcaattaataaataaatgagttgcatttaagacactttttt
tggaatatcaggcaaatattgtgtataaaaggaagtcaatattaacatatgtaaataattgaatctgcaaaacttaactttattgtgtaattttattgaa
ctgtatatatcagcagctgtattttctatatttagcagccaaagtgctttacttcctgctgaatgatccacatgcaatcaacatagttgacaatgttgta
atgtcactggggagcaaatggcactatagttaggtttgtcagcgctaggccaagacaagagtgagctttggtggggcattagaataggtaacaacaatta
tcttcataatataaatatgaagcgactgatgactgcatatataagcatacttttaaccatacgtaatgccattagtgttctttataaggatgttaatatt
t
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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