XB-CLONE-3058145
Clone Name: IMAGE:7847512
|
Gene:
prss29
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT531788 [+]
ggaggctgagcttagggaacgtagtaaattagcaaaacagcacatcaggtaatatctgctatagaagctgattaataattagaatcataatatgcagact
acactggttcttgtgttgccatgtaatggaatgtgggttttagagttttttttgcattgtttaatattttcccagctctccagagcagtggctgcataaa
tatgcaaaataatcctacaatgagaatcccagatgatctgtgtaaattttgttcctgcaattggagttgggagcattaagcacagtttcccagcgctgaa
caagtctgtccctttattcccatgtctgatttctgtgtcatataatgaaaggaaaatgacaattatctctatatataagatatcaaaatggctgctatag
tgccgggaatctaattagcattctcattggtcagttctcttgtgtatataattttttcttcaacctcgatagagaactaggtggaacctaaaaattaatc
cataaatgatacaggccaaatgatcccattggcacagaaacacaggtgtatcatggatacagttacacataaattagtactgtgctattatacacatttt
attacaagatatcaataaaaacaagctttagcttagtatttagacataacttgtttacaacaggctcccaaatatcatatctccatcacccaaaatatca
gctcataagataattgtggctttttctaaccaaaactcattctgaattctcttcttctacaaacaactttctgacacttgcattacaatgaccagagcaa
aggtttattgagcagactgggggtttcgtacacagtctgtttataatagtgagagcacaaactct
3' EST: DT531787 [+]agcagataggttttatctatgtactgtatgtatacaatgtatatcaagccccatgcccctgaagatgcccacagtagatctccatcttttgccgtgccca
actactgcacatgctcagtctgctcttggctgacctgaacttagggatcgactcacaatattcttctttctcctgcctccttgctctagtcactgctaaa
catgcatataagccaaccaacactgagttcagagtttgtgctctcactattataaacagactgtgtacgaaacccccagtctgctcaataaacctttgct
ctggtcattgtaatgcaagtgtcagaaagttgtttgtagaagaagagaattcagaatgagttttggttagaaaaagccacaattatcttatgagctgata
ttttgggtgatggagatatgatatttgggagcctgttgtaaacaagttatgtctaaatactaagctaaagcttgtttttattgatatcttgtaataaaat
gtgtataatagcacagtactaatttatgtgtaactgtatccatgatacacctgtgtttctgtgccaatgggatcatttggcctgtatcatttatggatta
atttttaggttccacctagttctctatcgaggttgaagaaaaaattatatacacaagagaactgaccaatgagaatgctaattagattcccggcactata
gcagccattttgatatcttatatatagagataattgtcattttcctttcattatatgacacagaaatcagacatgggaataaagggacagacttgttcag
cgctgggaaactgtgcttaatgctcccaac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: