XB-CLONE-3058097
Clone Name: IMAGE:7847564
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Gene:
zdhhc5
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531819 [+]
gaatatcgggactgctgggcggccctgagatacgtctaccaaaaatacatgttttgcttttgtactgcaaatgcccactagtacatttggattatcatca
aattgctagaatctacaggagagagaaacactggaaaaaggagcgttgtctgctttccataccaaataattgctagaggagggtagtggtggcaaacgta
ttagctcctgagttctcaagaaggaacagattacaaatcatcaagccttttccagttcttttttttctgaaatactttggattattcaacatgcctggtg
atagtttattgtatttgtttacctttttttgacattttttgtttacatacctcattcccactattctgtatttcatattacctaccaactttttatggtg
ctatgctgtacttcactgacgcctctctacatcttcacgatgcctgcagaaaatgttaagtcatttaaaccaagcaaatacgtacctgtggctgcagcaa
ctgtttttttggtgggatctacaacactgttcttcattttcacatgcccctggcttagtgtggaagtgtcctttgctctccctgcctataacgcagtcat
gtttctctttgtactggccaattttagcatggctactttcatggatcctggcatcttccctcgagcggaggaggatgaagataaagaagatgactttcgg
gcacctttatacaagacagtggaggtaaggggagtgcaggttcgcatgaagtggtgttcaacatgccgtttctatcgtccacctcggtgttcccactgca
gcgtttgtgacaattgtgttgaggagtttgatcaccattgtccc
3' EST: DT531818 [+]aaaattcataatcacttattattttaaatccactgtggtgaggacatgcttcccagattttgtctttcatgaagaaaggaaggccagattggcaaataaa
actcatgttgaacaaggggggtatgctgccccttttgagggtggaaattataactacatttaaatgcatacatgctagcagaaaggcggcgccatcttaa
agtgcagaaaattattttacaacaagagcaaaagaaacagattttgatttcaagaaaggactggataccataagtgagaaaattgctaacttgttccaga
gactggtctgtcctttctggagtcaagaagaaatcacacagaatcaagtttggaatggatgactagacagtcatacagtttagtcacctgcaatgtagtt
ttgacaaagaacattttgttcaacctgactcacattcctattgtacttttttttttaaaaataaaacaaagataattttctccttctgagtggactggat
atgtagtgtctgttctctctctctatatatatttgtatgtttgtgtgtataaatatacaataaacatacatatatttctgccagtaattatcacctgata
aagaaaagaaaatatgcattaaaccggaaagagacaccttaactttcaaatatgtaagagaattacattctatccaatactactaatcaggtgtcaccat
catccaagtattacatagaaatatactatgtcctataaactgattgcagcacaaaggtaaagcttgatttgcctctgcagtaaaaatgcttttctgccaa
tacaatttactaattattgaaccccagaaagacatccttattttgga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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