XB-CLONE-3057977
Clone Name: IMAGE:7847670
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531898 [+]
cgattcgcccgccacagggtgccccagttaagaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcccaccaggtccc
aatcaaatccaaaacataccaggttatgcaggagtgtgaacaatctggaacaacagccccttctgtgttcagccagaacaggacaggcggagaaacagcc
tttgacaagccaaaggaaggcccagcgaaaagtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatttttgttcccac
cttctactcattgtgctgcgcacatcatgcagagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagttactgtaagactt
tatttcattgctcctatggtcgggctgtgctgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctcttcagttattaag
tcaacaaaggagccaaatatgattttttagatttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaacccttacaggt
ccttaggctttggctggaccaaatcagaacacctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcacctactccccatcat
cagggtgatatggttcagtcctggcatttttcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgagggatcacctgcttt
aattgttgcattanatgtaaaattaggacttttcccngtaggtactcatccctaacaaactaagggctgat
3' EST: DT531897 [+]gcttgtacagactttgttcacaagtttaattgtaaaaccaggaaagtttaaaaaaaaaaaaccatataagaacatagcgacacaagaatgatcagggcta
atacacaacacacatttacttctcagtgatgctgtgactgggtgcactgttctacaaggtttcatttaatacaccctgacactatctagcaccccgagca
gaagcctagtgtgatgccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatcat
agaactaactggaacacagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattcact
tattgaatagaaggctacaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagttg
gagcctataaaggttgctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaagc
aggtgatccctcaccaggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccaggactgaaccatatcaccctgatgat
ggggagtaggtgatatgactgtgtttaggtccccagcccagtaaagtcacatgacgaanagaggtgttctgatttggtccagccaaagcctaaggacctg
taagggtttggattttagaaaatacaaattctgtattc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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