XB-CLONE-3057922
Clone Name: IMAGE:7847436
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Gene:
cnih1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT531934 [+]
aaaacagactacaaaaatcctatagaccagtgtaacacactcaaccctcttgtactccctgagtatctgattcatgctttcttctgtgtcatgtatctgt
gtgctgcggagtggctaaccttgggtttaaacatgcctcttttggcttatcatatttggaggtaatgtgtaaaactgacatttttgaattatacatggaa
gtttatattctgttgcttcatatttatatggtgtcatattttttttttcttttataggtatatgagtaggccggtcatgagtggaccaggattgtatgat
cctacaaccattatgaatgctgatatactagcttactgtcaaaaggaagggtggtgcaaattagctttttaccttctgtcatttttttactacctgtacg
ggtaagttgtgtttatgtggagctgctgttttcatgctgtactatattttataaaagaaaaatgctatgcaagactcctgcagatgtttttggaatttta
ttgcttcttgtatcatttattatttgttatcactgtttcatttttttacataatgttttgtatgagttaaggaatcttgcaatagcaacagtataggctg
tatacactggaacttgtgtataagaataaataatgcacccccttttgtaatatataaggatattagaagtcacctcggaattccaaaacctgtataaaag
tgctctgcctttggccttgtgcttttttatggggttaagtcccagaatccatcatgtcaggatgggagactggtagtattccataatggaatatggaaaa
tggttagtcatgatatcatggtttcctttctngtaagtcaggtaggtttgcgtaaaaaaaaagc
3' EST: DT531933 [+]ttttttttacgcaaacctacctgacttaacagaaaggaaaccatgatatcatgactaaccattttccatattccattatggaatactaccagtctcccat
cctgacatgatggattctgggacttaaccccataaaaaagcacaaggccaaaggcagagcacttttatacaggttttggaattccgaggtgacttctaat
atccttatatattacaaaagggggtgcattatttattcttatacacaagttccagtgtatacagcctatactgttgctattgcaagattccttaactcat
acaaaacattatgtaaaaaaatgaaacagtgataacaaataataaatgatacaagaagcaataaaattccaaaaacatctgcaggagtcttgcatagcat
ttttcttttataaaatatagtacagcatgaaaacagcagctccacataaacacaacttacccgtacaggtagtaaaaaaatgacagaaggtaaaaagcta
atttgcaccacccttccttttgacagtaagctagtatatcagcattcataatggttgtaggatcatacaatcctggtccactcatgaccggcctactcat
atacctataaaagaaaaaaaaaatatgacaccatataaatatgaagcaacagaatataaacttccatgtataattcaaaaatgtcagttttacacattac
ctccaaatatgataagccaaaagaggcatgtttaaacccaaggttagccactccgcagcacacagatacatgacacagaagaaagcatgaatcagatact
cagggagtacaagagggttgagtgtgttacactggtctataggatttttgtagtctgtttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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